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Series GSE17020 Query DataSets for GSE17020
Status Public on Aug 01, 2009
Title Genome-wide analysis of murine cardiomyocytes at different developmental stages
Organism Mus musculus
Experiment type Expression profiling by array
Summary Cardiomyocytes exhibit differential growth patterns throughout development. In fetal life the increase in cardiac mass is associated with hyperplastic growth and cardiomyocyte proliferation. The majority of fetal cardiomyocytes are also mononucleated. During the early neonatal period in mice there is a switch from hyperplastic to hypertrophic growth of cardiomyocytes. This period is characterized by bi-nucleation and polyploidization of cardiomyocyte nuclei and a decreased capacity for cardiomyocytes to proliferate and complete cytokinesis. Increases in myocardial mass occur predominantly via hypertrophic growth. Adult mammalian cardiomyocytes are generally accepted to have little or no proliferative capacity and to be terminally withdrawn from the cell cycle. The vast majority of adult murine cardiomyocytes are bi-nucleated. The present study sought to accurately establish the growth pattern of cardiomyocytes throughout development in mice and identify genes associated with the switch from hyperplastic to hypertrophic growth. These cell cycle associated genes are crucial to the understanding of the mechanisms of bi-nucleation, polyploidization and hypertrophy in the neonatal period. Cardiomyocytes were FACS sorted from the hearts of ED11-12 embryos, neonatal day 3-4 and adult (10 week) eGFP ?-MHC mice whereby GFP expression is driven constitutively by the ?-MHC promoter. Gene analyses identified genes whose expression was predicted to be particular to day 3 -4 neonatal cardiomyocytes, compared to embryonic or adult cells. Cell cycle associated genes are crucial to the understanding of the mechanisms of bi-nucleation and hypertrophy in the neonatal period, and offer attractive candidates for manipulation.
Overall design Total RNA obtained from isolated cardiomyocytes from ED11-12; Neonatal day 3-4 and adult timepoints compared with each other. Several hearts per sample, RNA was pooled within samples. FACS samples were prepared in the following manner: embryonic, neonatal and adult hearts were dissected and dissociated to single cell solution with Liberase Blendzyme 3 (0.1 mg/ml) (Roche Diagnostics), washed and spun down and resuspended in cardiomyocyte isolation buffer (130 mM NaCl; 5 mM KCl; 1.2 mM KH2PO4; 6 mM HEPES; 5 mM NaHCO3; 1 mM MgCl2; 5 mM Glucose).
Contributor(s) Walsh S, Pontén A
Citation(s) 20071355
Submission date Jul 08, 2009
Last update date Mar 21, 2012
Contact name Stefan Jovinge
Organization name Lund Strategic Center for Stem Cell Biology and Cell Therapy
Street address Klinikgatan 26
City Lund
ZIP/Postal code 221 84
Country Sweden
Platforms (1)
GPL8833 Lund Strategic Center Mouse Illumina Expression Chip V6.1.1
Samples (3)
GSM425743 ED11-12 embryonic cardiomyocytes
GSM425744 Neonatal day3-4
GSM425745 Adult 10 weeks
BioProject PRJNA117769

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17020_raw.txt.gz 3.1 Mb (ftp)(http) TXT
GSE17020_sample_comparisons.txt.gz 910.7 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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