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Series GSE1709 Query DataSets for GSE1709
Status Public on Aug 25, 2005
Title STS107 T-cell activation at 1g vs. vg
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Peripheral blood leukocytes of three human donors (Donors 2, 4, and 5) were isolated from blood bank buffy-coat preparations by Ficoll-Hypaque density gradient centrifugation, and T-cells were purified with high affinity negative selection human CD3+ T-cell enrichment columns. The cells were resuspended in RPMI-1640 with 10% FCS with approximately 3-8 millions cells/mL at room temperature overnight. T-cells were then either unactivated (1g 0 hr), activated (1g 4 hr) with a final concentration of 5 mg/mL Con A + 4 mg/mL anti-CD28 antibody and incubated for 4 hours at 37°C, or loaded onto a RPM rotating at 60°/s (vg). T-cells loaded on the RPM were cultured for 2 hours prior to collection or activation to allow equilibration of cells to the new environment. After incubation, 1 mL of 4 M guanidinium isothiocyanate in 750 mM sodium citrate buffer, pH 7, with N-lauroylsarcosine and beta-mercaptoethanol was added to lyse cells and stabilize RNA.
Keywords: ordered
Contributor(s) Boonyaratanakornkit JB
Citation(s) 16210397
Submission date Aug 25, 2004
Last update date Jul 08, 2016
Contact name Jim Boonyaratanakornkit
Phone 301-594-2589
Organization name NIH - NIAID
Department LID
Street address 50 South Dr, Bldg 50, Rm 6513
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL201 [HG-Focus] Affymetrix Human HG-Focus Target Array
Samples (12)
GSM24817 Donor 2 1g 0 hr
GSM29564 Donor 2 1g 4 hr
GSM29565 Donor 2 vg 0 hr
BioProject PRJNA114695

Download family Format
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Supplementary file Size Download File type/resource
GSE1709_RAW.tar 14.1 Mb (http)(custom) TAR (of CEL, EXP, RPT)

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