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Series GSE171407 Query DataSets for GSE171407
Status Public on Mar 30, 2022
Title The TH1 cell lineage-determining transcription factor T-bet supresses TH2 gene expression by redistributing GATA3 away from TH2 genes [ATAC-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Differentiation of progenitor cells into mature cell types is commonly associated with the expression of lineage-determining transcription factors (LD-TFs) specific to that lineage. In CD4+ T cells, T-bet dictates differentiation of the TH1 lineage, whereas GATA3 drives differentiation of the alternative TH2 lineage. LD-TFs, including T-bet and GATA3, are frequently co-expressed, both in vitro and in vivo. How co-expression of two mutually antagonistic LD-TFs affects their function and lineage determination is not known. By expressing T-bet and GATA3 separately or together, we show that T-bet sequesters GATA3 at its target sites, thereby removing GATA3 from TH2 genes. T-bet interacts with the GATA3 DNA binding domain, changing its DNA sequence binding preference. This mechanism allows T-bet to dominate and drive the TH1 gene expression program in the presence of the GATA3. We propose that redistribution of one LD-TF by another may be a common mechanism that could explain how specific cell fate choices can be made even in the presence of other TFs driving alternative differentiation pathways.
Overall design ATAC-seq in unstimulated and stimulated in FLAG-T-bet expressing EL4 cells and control cells not expressing T-bet
Contributor(s) Hertweck A, Jenner RG
Citation(s) 35438764
Submission date Apr 02, 2021
Last update date May 11, 2022
Contact name Arnulf Hertweck
Organization name University College London
Department UCL Cancer Institute
Street address Gower Street
City London
ZIP/Postal code WC1E 6BT
Country United Kingdom
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (4)
GSM5224982 EL4 GFP cells unstimulated
GSM5224983 EL4 GFP cells stimulated
GSM5224984 EL4 T-bet cells unstimulated
This SubSeries is part of SuperSeries:
GSE171410 The TH1 cell lineage-determining transcription factor T-bet supresses TH2 gene expression by redistributing GATA3 away from TH2 genes
BioProject PRJNA719405
SRA SRP313270

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Supplementary file Size Download File type/resource
GSE171407_RAW.tar 778.0 Mb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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