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Series GSE171459 Query DataSets for GSE171459
Status Public on Apr 06, 2021
Title A RALF-like peptide EaF82 derived from Epipremnum aureum inhibits pollen development in Arabidopsis and tobacco
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary To investigate the function of previously cloned novel cysteine-rich peptide EaF82 which biochemical properties are similar to the Rapid Alkalinization Factor (RALF), we overexpressed EaF82 gene in Arabidopsis and found defects only in the pollen development. To further determine the affected genes and biological pathways, we compared the transcriptome profilings of two independent Arabidopsis EaF82 overexpressing lines (TA and TB) with that of the vector control line (C). The RNAseq analysis results revealed decreased expressions of five endogenous clade IV AtRALFs and genes responsible for cell wall modifications and pollen maturation, supporting the observed pollen abortion.
 
Overall design The clusters of unopened flower buds of two indepentdent transgenic lines overexpressing EaF82, named TA and TB, and one control line expressing vector contruct, named C, were examined. Each line has three biological replicate samples randomly collected from 36 subline plants.
 
Contributor(s) Hung C, Xie J
Citation(s) 37296662
Submission date Apr 05, 2021
Last update date Sep 07, 2023
Contact name Jiahua Xie
E-mail(s) jxie@nccu.edu
Phone 919-530-6705
Organization name North Carolina Central University
Department Pharmaceutical Sciences
Lab Jiahua Xie Laboratory
Street address 302 E. Lawson Street
City Durham
State/province NC
ZIP/Postal code 27707
Country USA
 
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (9)
GSM5225908 EaF82_OX_1-TA1
GSM5225909 EaF82_OX_1-TA2
GSM5225910 EaF82_OX_1-TA3
Relations
BioProject PRJNA719729
SRA SRP313445

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE171459_FPKM_EaF82.txt.gz 1.4 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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