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Series GSE172023 Query DataSets for GSE172023
Status Public on Oct 02, 2023
Title RNAseq of JAK2V617F-positive MPN cell lines after 4-Hydroxytamoxifen treatment
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Gene expression profile in HEL/UKE-1 cells after 4-hour 4-Hydroxytamoxifen treatment
 
Overall design For RNA-Seq of JAK2V617F-mutated human cells lines, three cells lines (HEL cells, UKE-1 with and without Horse Serum, UKE-1+/-HS respectively) were treated with 10 μM tamoxifen or vehicle for 4 h and RNA was extracted from tamoxifen-treated cells by Trizol and cleaned-up with RNeasy Mini Columns . RNA-Seq libraries were prepared from total RNA using poly(A) enrichment of the mRNA and sequenced by HiSeq 4000.
 
Contributor(s) Fang Z, Fattori GC, McKerrell T, Boucher RH, Jackson A, Fletcher RS, Martin J, Fox S, Roberts J, Glover R, Harris E, Grassi L, Rodriguez-Meira A, Mead AJ, Knapper S, Ewing J, Butt NM, Jain M, Francis S, Clark FJ, Coppell J, McMullin MF, Wadelin F, Narayanan S, Milojkovic D, Drummond MW, Sekhar M, Paramor M, Baxter EJ, Godfrey AL, Harrison CN, Méndez-Ferrer S
Citation(s) 38001082
Submission date Apr 13, 2021
Last update date Dec 14, 2023
Contact name Irina Mohorianu
E-mail(s) data-submissions@stemcells.cam.ac.uk
Organization name University of Cambridge
Department Wellcome-MRC Cambridge Stem Cell Institute
Street address Puddicombe Way
City Cambridge
ZIP/Postal code CB2 0AW
Country United Kingdom
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (18)
GSM5240033 HEL control 1
GSM5240036 HEL control 2
GSM5240038 HEL control 3
This SubSeries is part of SuperSeries:
GSE172025 RNA-seq of Myeloproliferative neoplasms primary CD34+ HSPCs and cell lines after tamoxifen treatment
Relations
BioProject PRJNA721719
SRA SRP314719

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE172023_norm.csv.gz 2.0 Mb (ftp)(http) CSV
GSE172023_raw.csv.gz 896.1 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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