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| Status |
Public on Oct 02, 2023 |
| Title |
RNAseq of JAK2V617F-positive MPN cell lines after 4-Hydroxytamoxifen treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Gene expression profile in HEL/UKE-1 cells after 4-hour 4-Hydroxytamoxifen treatment
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| Overall design |
For RNA-Seq of JAK2V617F-mutated human cells lines, three cells lines (HEL cells, UKE-1 with and without Horse Serum, UKE-1+/-HS respectively) were treated with 10 μM tamoxifen or vehicle for 4 h and RNA was extracted from tamoxifen-treated cells by Trizol and cleaned-up with RNeasy Mini Columns . RNA-Seq libraries were prepared from total RNA using poly(A) enrichment of the mRNA and sequenced by HiSeq 4000.
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| Contributor(s) |
Fang Z, Fattori GC, McKerrell T, Boucher RH, Jackson A, Fletcher RS, Martin J, Fox S, Roberts J, Glover R, Harris E, Grassi L, Rodriguez-Meira A, Mead AJ, Knapper S, Ewing J, Butt NM, Jain M, Francis S, Clark FJ, Coppell J, McMullin MF, Wadelin F, Narayanan S, Milojkovic D, Drummond MW, Sekhar M, Paramor M, Baxter EJ, Godfrey AL, Harrison CN, Méndez-Ferrer S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 13, 2021 |
| Last update date |
Oct 02, 2023 |
| Contact name |
Irina Mohorianu |
| E-mail(s) |
data-submissions@stemcells.cam.ac.uk
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| Organization name |
University of Cambridge
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| Department |
Wellcome-MRC Cambridge Stem Cell Institute
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| Street address |
Puddicombe Way
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| City |
Cambridge |
| ZIP/Postal code |
CB2 0AW |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE172025 |
RNA-seq of Myeloproliferative neoplasms primary CD34+ HSPCs and cell lines after tamoxifen treatment |
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| Relations |
| BioProject |
PRJNA721719 |
| SRA |
SRP314719 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE172023_norm.csv.gz |
2.0 Mb |
(ftp)(http) |
CSV |
| GSE172023_raw.csv.gz |
896.1 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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