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Series GSE172387 Query DataSets for GSE172387
Status Public on Apr 19, 2024
Title Loss of retinoic acid signaling drives immune escape in IDH mutant gliomas
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Gliomas are immunologically cold tumors that can be broken into several categories based on either RNA expression profiles or methylation profiles, with isocitrate dehydrogenase (IDH) mutations defining a major segregration between types. IDH mutant gliomas often exhibit defects in the retinoic acid pathway. We treated mice harboring IDH mutant gliomas with all-trans retinoic acid, and found that this treatment cause reductions in tumor growth and a swith in immune profiles in the tumor microenvironment.
Overall design Neurospheres (50,000 cells) were injected into the striatium of 6 week old mice. Mice were treated with either 10 mg/kg ATRA or control intra-peritoneally every other day until they were sacrificed based on signs of CNS pathology. 5-prime based single-cell RNAseq was then performed on manually dissected and digested tumors.
Contributor(s) Cillo AR, Rao A, Zhang X, Bruno TC, Vignali DA, Amankulor N
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Submission date Apr 20, 2021
Last update date Apr 19, 2024
Contact name Nduka Amankulor
Organization name University of Pittsburgh
Department Neurological Surgery
Street address 200 Lothrop Street
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM5253930 murine_glioma_control_gex
GSM5253931 murine_glioma_ATRA_gex
GSM5253932 murine_glioma_control_tcr
BioProject PRJNA723194
SRA SRP315506

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Supplementary file Size Download File type/resource
GSE172387_RAW.tar 10.5 Mb (http)(custom) TAR (of CSV, TAR)
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Raw data are available in SRA
Processed data provided as supplementary file

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