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| Status |
Public on Jan 01, 2010 |
| Title |
MYC Transactome Mapped by Global Array-based Nuclear Run-on (ANRO - Affymetrix) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We describe and illustrate the use of a method to measure nascent nuclear gene transcription with an Array-based Nuclear Run-On (ANRO) assay using commercial microarray platforms.
ANRO measurements in an inducible c-Myc expressing human P493-6 B cell model was performed following 48 hours with or without MYC gene induction. Samples were prepared from either nuclear run-on or total RNA and were applied to Affymetrix Exon arrays and similarly regulated, full length transcripts were demonstrated from each preparation type.
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| |
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| Overall design |
Gene expression is compared at a global level after 48 hours with and without tetracycline as measured for both nuclear run-on and total RNA using the Affymetrix Exon microarray platform.
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| |
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| Contributor(s) |
Fan J, Cheadle C |
| Citation(s) |
20300622 |
| |
| Submission date |
Jul 21, 2009 |
| Last update date |
Feb 18, 2019 |
| Contact name |
chris cheadle |
| E-mail(s) |
ccheadl1@jhmi.edu
|
| Phone |
4105505984
|
| Organization name |
JHU
|
| Department |
SOM
|
| Lab |
JHBMC Genomics Core
|
| Street address |
5200 Eastern Ave
|
| City |
baltimore |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
|
| Samples (16)
|
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| Relations |
| BioProject |
PRJNA118959 |
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