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| Status |
Public on Jun 06, 2023 |
| Title |
Costs of reproduction and their effect on age-related gene expression in Bombus terrestris queens |
| Organism |
Bombus terrestris |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Our two main aims were 1) to isolate age-related changes in gene expression in queens of the eusocial bumble bee, Bombus terrestris; and 2) to determine whether experimentally increasing the costs of reproduction (by removing eggs) caused changes in age-related gene expression in these queens. To address these aims we extracted RNA from three key tissues (brain, fat body and ovary) from queens at two time points (10% and 60% mortality phases). Each of these queens had experienced one of two treatments: an egg removal (R) treatment (to increase the costs of reproduction) and an egg removal and replacement (C) treatment (to control for the effects of disturbance caused by egg removal).
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| Overall design |
We used mRNA-seq to identify the RNA profiles in three tissue types (brain, fat body and ovary) in two time points (after 10% cohort death (TP1) and 60% cohort death (TP2)) for two treatments (egg removal (R) and egg removal and replacement (C)), with up to six biological replicates per tissue/time point/treatment combination. Each sample was sequenced on two lanes (L1 and L2) of the sequencing machine in order to generate sufficient numbers of reads while controlling for between lane variability.
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| Web link |
https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-023-01648-5
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| Contributor(s) |
Collins DH, Prince DC, Donelan J, Chapman T, Bourke AF |
| Citation(s) |
37430246 |
| Submission date |
Apr 20, 2021 |
| Last update date |
Jul 12, 2023 |
| Contact name |
David C. Prince |
| Organization name |
University of East Anglia
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| Street address |
Norwich Research Park
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| City |
Norwich |
| ZIP/Postal code |
NR4 7TJ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL30019 |
Illumina NovaSeq 6000 (Bombus terrestris) |
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| Samples (70)
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| Relations |
| BioProject |
PRJNA723286 |
| SRA |
SRP315556 |
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