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Series GSE172422 Query DataSets for GSE172422
Status Public on Jun 06, 2023
Title Costs of reproduction and their effect on age-related gene expression in Bombus terrestris queens
Organism Bombus terrestris
Experiment type Expression profiling by high throughput sequencing
Summary Our two main aims were 1) to isolate age-related changes in gene expression in queens of the eusocial bumble bee, Bombus terrestris; and 2) to determine whether experimentally increasing the costs of reproduction (by removing eggs) caused changes in age-related gene expression in these queens. To address these aims we extracted RNA from three key tissues (brain, fat body and ovary) from queens at two time points (10% and 60% mortality phases). Each of these queens had experienced one of two treatments: an egg removal (R) treatment (to increase the costs of reproduction) and an egg removal and replacement (C) treatment (to control for the effects of disturbance caused by egg removal).
Overall design We used mRNA-seq to identify the RNA profiles in three tissue types (brain, fat body and ovary) in two time points (after 10% cohort death (TP1) and 60% cohort death (TP2)) for two treatments (egg removal (R) and egg removal and replacement (C)), with up to six biological replicates per tissue/time point/treatment combination. Each sample was sequenced on two lanes (L1 and L2) of the sequencing machine in order to generate sufficient numbers of reads while controlling for between lane variability.
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Contributor(s) Collins DH, Prince DC, Donelan J, Chapman T, Bourke AF
Citation(s) 37430246
Submission date Apr 20, 2021
Last update date Jul 12, 2023
Contact name David C. Prince
Organization name University of East Anglia
Street address Norwich Research Park
City Norwich
ZIP/Postal code NR4 7TJ
Country United Kingdom
Platforms (1)
GPL30019 Illumina NovaSeq 6000 (Bombus terrestris)
Samples (70)
GSM5255710 brain_C_TP1G_rep1
GSM5255711 brain_C_TP1G_rep2
GSM5255712 brain_C_TP1G_rep3
BioProject PRJNA723286
SRA SRP315556

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Supplementary file Size Download File type/resource
GSE172422_NER0008751_obj1_exp1_brain_all_expressed_genes.csv.gz 1.3 Mb (ftp)(http) CSV
GSE172422_NER0008751_obj1_exp1_fatbody_all_expressed_genes.csv.gz 1.3 Mb (ftp)(http) CSV
GSE172422_NER0008751_obj1_exp1_ovary_all_expressed_genes.csv.gz 1.4 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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