GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE173075 Query DataSets for GSE173075
Status Public on Sep 27, 2023
Title Single-Cell Multiomics Reveals Distinct Cell States at the Top of the Human Hematopoietic Hierarchy [scATAC-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The advent of single cell (Sc) genomics has challenged the dogma of haematopoiesis as a tree-like structure of stepwise lineage commitment through distinct and increasingly restricted progenitor populations. Instead, analysis of ScRNA-seq has proposed that the earliest events in human hematopoietic stem cell (HSC) differentiation are characterized by only subtle molecular changes, with hematopoietic stem and progenitor cells (HSPCs) existing as a continuum of low-primed cell-states that gradually transition into a specific lineage (CLOUD-HSPCs). Here, we combine ScRNA-seq, ScATAC-seq and cell surface proteomics to dissect the heterogeneity of CLOUD-HSPCs at different stages of human life. Within CLOUD-HSPCs, pseudotime ordering of both mRNA and chromatin data revealed a bifurcation of megakaryocyte/erythroid and lympho/myeloid trajectories immediately downstream a subpopulation with an HSC-specific enhancer signature. Importantly, both HSCs and lineage-restricted progenitor populations could be prospectively isolated based on correlation of their molecular signatures with CD35 and CD11A expression, respectively. Moreover, we describe the changes that occur in this heterogeneity as hematopoiesis develops from neonatal to aged bone marrow, including an increase of HSCs and depletion of lympho-myeloid biased MPPs. Thus, this study dissects the heterogeneity of human CLOUD-HSPCs revealing distinct HSPC-states of relevance in homeostatic settings such as ageing.
Overall design Singel-Cell ATAC sequencing of hematopoietic stem and progenitors of young bone marrow.
*** The raw data cannot be shared due to EU law (derived from EU patients that are protected by GDPR) ***
Contributor(s) Sommarin MN, Dhapola P, Karlsson G
Citation(s) 35545037
Submission date Apr 22, 2021
Last update date Sep 28, 2023
Contact name Göran Karlsson
Phone +46 462221261
Organization name Lund University
Department Division of Molecular Hematology
Lab Stem cells and leukemia lab
Street address Kilinkgatan 26 BMC B12
City Lund
State/province Scania
ZIP/Postal code 221 84
Country Sweden
Platforms (1)
GPL24268 Illumina Nextseq 500 (Homo sapiens)
Samples (10)
GSM5259374 yBM_hpc_scATAC-seq
GSM5259375 yBM_hsc_scATAC-seq
GSM5259376 yBM_CD35_scATAC-seq
This SubSeries is part of SuperSeries:
GSE173077 Single-Cell Multiomics Reveals Distinct Cell States at the Top of the Human Hematopoietic Hierarchy
BioProject PRJNA723752

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE173075_RAW.tar 2.6 Gb (http)(custom) TAR (of ZIP)
Raw data not provided for this record
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap