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| Status |
Public on Apr 30, 2023 |
| Title |
Expression data from murine BM Tx transduced with BRSST gRNA +/- sgEzh2 [array] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Mice transplanted with BRSST (Bcor, Runx1, Sh2b3, Stag2, Tet2) sgRNAs in addition to the sgEzh2 develop myeloid leukemia with a significantly earlier onset compared to missing sgLuc.
We used global expression analysis by microarray to determine regulated gene programs after addition of the Ezh2 sgRNA.
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| Overall design |
LSK cells expressing SpCas9 were transduced with the sgRNA combination and transplanted into irradiated C57Bl/6J mice. BM was harvested after development of a leukemia or after 6 month and used for RNA extraction.
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| Contributor(s) |
Borchert D, Heckl D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 22, 2021 |
| Last update date |
May 02, 2023 |
| Contact name |
Dorit Borchert |
| E-mail(s) |
Borchert.Dorit@mh-hannover.de
|
| Organization name |
Hannover Medical School
|
| Department |
Pediatric Hematology and Oncology
|
| Street address |
Carl-Neuberg-Str. 1
|
| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (13)
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| This SubSeries is part of SuperSeries: |
| GSE173497 |
Oncofetal gene reactivation drives EZH2-mutant myeloid leukemia pathogenesis |
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| Relations |
| BioProject |
PRJNA723866 |
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