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Series GSE173135 Query DataSets for GSE173135
Status Public on Apr 30, 2023
Title Expression data from murine BM Tx transduced with BRSST gRNA +/- sgEzh2 [array]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mice transplanted with BRSST (Bcor, Runx1, Sh2b3, Stag2, Tet2) sgRNAs in addition to the sgEzh2 develop myeloid leukemia with a significantly earlier onset compared to missing sgLuc.
We used global expression analysis by microarray to determine regulated gene programs after addition of the Ezh2 sgRNA.
Overall design LSK cells expressing SpCas9 were transduced with the sgRNA combination and transplanted into irradiated C57Bl/6J mice. BM was harvested after development of a leukemia or after 6 month and used for RNA extraction.
Contributor(s) Borchert D, Heckl D
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Submission date Apr 22, 2021
Last update date May 02, 2023
Contact name Dorit Borchert
Organization name Hannover Medical School
Department Pediatric Hematology and Oncology
Street address Carl-Neuberg-Str. 1
City Hannover
ZIP/Postal code 30625
Country Germany
Platforms (1)
GPL23038 [Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay)
Samples (13)
GSM5260619 BRSST-Ezh2 rpl1
GSM5260620 BRSST-Ezh2 rpl2
GSM5260621 BRSST-Ezh2 rpl3
This SubSeries is part of SuperSeries:
GSE173497 Oncofetal gene reactivation drives EZH2-mutant myeloid leukemia pathogenesis
BioProject PRJNA723866

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE173135_RAW.tar 15.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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