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Series GSE17319 Query DataSets for GSE17319
Status Public on Jul 25, 2009
Title Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary In germ cells, Piwi proteins interact with a specific class of small non-coding RNAs, piwi-interacting RNAs (piRNAs). Together, these form a pathway that represses transposable elements, thus safeguarding germ cell genomes. While basic models describe the operation of piRNA pathways, neither the protein compositions of Piwi complexes, the critical protein-protein interactions that drive small RNA production and target recognition, or the precise molecular consequences of conserved localization to germline structures, call nuage, is well understood. We purified the three murine Piwi family proteins, Mili, Miwi, and Miwi2, from mouse germ cells and characterized their interacting protein partners. Piwi proteins were found in complex with Prmt5/Wdr77, an enzyme that di-methylates arginine residues. By immunoprecipitation with specific antibodies and by mass spectrometry, we found that Piwi proteins are arginine methylated at conserved positions in their amino termini. These modifications are essential to direct complex formation with specific Tudor-domain proteins, whose interactions with Piwis can be required for localization of RNP complexes in cytoplasmic nuage, proper piRNA expression, and transposon silencing. Considered together, our findings indicate that arginine methylation drives the assembly of multi-protein machines whose integrity and specific sub-cellular localization is necessary for efficient function of the piRNA pathway.

Keywords: gene regulation study
 
Overall design Total small RNA in embryonic and post-birth mouse testes of tdrd1 and tdrd6 mutants
 
Contributor(s) Vagin VV, Wohlschlegel J, Qu J, Jonsson Z, Huang X, Chuma S, Girard A, Sachidanandam R, Hannon GJ, Aravin AA
Citation(s) 19584108, 26115953
Submission date Jul 24, 2009
Last update date May 15, 2019
Contact name Vasily Vagin
E-mail(s) vagin@cshl.edu
Organization name Cold Spring Harbor
Lab Hannon's
Street address 1 Bungtown Rd
City Cold Spring Harbor
State/province NY
ZIP/Postal code 11724
Country USA
 
Platforms (1)
GPL9185 Illumina Genome Analyzer (Mus musculus)
Samples (8)
GSM433288 18dpp_hetero_tdrd6-KO
GSM433289 18dpp_homo_tdrd6-KO
GSM433290 25dpp_hetero_tdrd6-KO
Relations
SRA SRP001307
BioProject PRJNA119045

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Supplementary file Size Download File type/resource
GSE17319_sequence_annotations.txt.gz 42.1 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Processed data included within Sample table
Raw data are available in SRA

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