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| Status |
Public on Jul 28, 2009 |
| Title |
Transcriptional termini of genes on chromosomes 21-22 (RNA mapping) |
| Project |
ENCODE
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| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
The purpose of these experiments was to determine the 5' and 3' transcriptional termini of genes on chromosomes 21-22. Towards this end, we mapped the poly A + RNA isolated from 12 normal tissues and four cell lines.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
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| Overall design |
polyA+ RNAs from 11 tissues (Brain Frontal Lobe, Brain Hippocampus, Brain Hypothalamus, Cerebellum, Ovary, Placenta, Prostate, Testis, Fetal Kidney, Fetal Spleen, Fetal Thymus; all from BD Clontech) and 5 cell lines (GM06990, HeLaS3, HepG2, K562, tert-BJ) using the BD SMARTTM RACE cDNA amplification kit (BD Clontech Cat. No.634914). A single array with no replicates was run for each sample with a pool of ~23-24 RACE reactions per sample.
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| Web link |
http://www.ncbi.nlm.nih.gov/projects/geo/info/ENCODE.html
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| Contributor(s) |
Gingeras T, Zaleski C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| BioProject |
PRJNA30709 |
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| Submission date |
Jul 27, 2009 |
| Last update date |
Jun 28, 2012 |
| Contact name |
Chris Zaleski |
| Organization name |
Cold Spring Harbor Labs
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| Lab |
Gingeras
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| Street address |
500 Sunnyside Blvd
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| City |
Woodbury |
| State/province |
NY |
| ZIP/Postal code |
11797 |
| Country |
USA |
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| Platforms (1) |
| GPL5715 |
AFFYMETRIX Human Chromosomes 21 & 22 v2.0 |
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| Samples (16)
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| Supplementary file |
Size |
Download |
File type/resource |
| GSE17358_RAW.tar |
1.1 Gb |
(http)(custom) |
TAR (of BAR, CEL) |
| Processed data provided as supplementary file |
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