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| Status |
Public on Mar 27, 2023 |
| Title |
10x Single-cell Gene expression analysis of pMHC-NP-induced Tet+ cells and anti-CD3-induced IL-10-producing cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To further explore the transcriptional homogeneity of Tet+ cells with high numbers, we performed scRNAseq of FACS-sorted BDC2.5mi/IAg7 and InsB13-21/IAg7 Tet+ and Tconv cells from pMHCII-NP-treated animals, using the 10x Genomics platform. To study the effect of Bcl6-KO, we also studied transcriptional differences between WT and Bcl6-KO a-CD3-induced IL-10-producing T cells
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| Overall design |
Treat NOD mice with BDC2.5mi/IAg7s or InsB13-21/IAg7-NPs to obtain Tet+ and Tconv T cells and perform scRNAseq with 10x technology and treat NOD or NOD CD4.cre.Bcl6(fl/fl) with a-CD3 antibody to obtain IL-10-producing CD4+ T cells and perform scRNAseq
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| Contributor(s) |
Pere SV, Patricia SS, Garnica J |
| Citation(s) |
36973489 |
| Submission date |
May 05, 2021 |
| Last update date |
Mar 31, 2023 |
| Contact name |
Pere Santamaria |
| E-mail(s) |
pesantam@clinic.cat
|
| Organization name |
IDIBAPS
|
| Street address |
C/Rosselló 149-153
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA727604 |
| SRA |
SRP318633 |
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