Expression profiling by high throughput sequencing
Summary
Chromatin remodeling and protein synthesis are tightly regulated processes that impact gene expression and cellular phenotypes. However, it is unknown if these two regulatory mechanisms are linked or exert independent effects in normal epithelial physiology and disease states. We have uncovered a new functional relationship between the chromatin remodeler ARID1A and mRNA translation elongation that is involved in cancer initiation and progression.
Overall design
Polysome to Subpolysome Profiling and RNASeq of samples