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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 12, 2021 |
| Title |
Cytokine profile of mature WT bone marrow-derived dendritic cells and fascin1 knockout bone marrow-derived dendritic cells |
| Organism |
Mus musculus |
| Experiment type |
Protein profiling by protein array
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| Summary |
Cytokines secreted by WT and fascin1 KO BM-DCs maturated by LPS were determined by RayBio Quantibody mouse Th17 array1. Fascin1 KO BM-DCs show reduced secretion of several cytokines including IL-6.
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| Overall design |
For analyses of cytokines in culture supernatants of BMDCs, WT and fascin1 KO BMDCs were cultured overnight after addition of LPS (100ng/ml). Culture supernatants were then used for profiling of inflammatory cytokines using a RayBio Quantibody mouse Th17 array1 according to the manufacturer’s instructions (RayBiotech). The Quantibody array allows us to quantitatively determine the concentrations of 18 cytokines/chemokines (IL-1, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 p70, IL-17, IL17F, IL-21 IL-22, IL-23, IL-28, IFN, MIP-3, TGF1, and TNF).
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| Contributor(s) |
Matsumura F, Yamashiro S |
| Citation(s) |
34301846 |
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| Submission date |
May 11, 2021 |
| Last update date |
Aug 11, 2021 |
| Contact name |
Fumio Matsumura |
| E-mail(s) |
matsumura@dls.rutgers.edu
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| Organization name |
Rutgers
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| Department |
MBB
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| Street address |
604 Allison Rd
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| City |
Piscataway |
| ZIP/Postal code |
08854 |
| Country |
USA |
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| Platforms (1) |
| GPL30121 |
RayBio Quantibody Mouse Th1/Th2/Th17 Array Q1 |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA729095 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE174285_RAW.tar |
10.0 Kb |
(http)(custom) |
TAR (of TXT) |
| GSE174285_normalized.txt.gz |
661 b |
(ftp)(http) |
TXT |
| Processed data are available on Series record |
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