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| Status |
Public on Jun 30, 2023 |
| Title |
Next generation sequencing facilitates quantitative analysis of colonic tissue from miR-7 deficiency and WT mice with IBD mice, and human colonic epithelial cells transfected-with NC/miR-7 inhibitor/miR-7 inhibitor plus Erlotinib following LPS treatment |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Methods: Colon tissue from 8-10-Week wild-type (WT) and miR-7 deficiency mice with DSS treatment, and miR-7 inhibitor/NC-transfected-FHC cells and Erlotinib plus miR-7 inhibitor-transfected-FHC cells with LPS treatment, mRNA profiles of which were generated by deep sequencing, using Illumina. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays
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| Overall design |
mRNA profiles of colon tissue from 8-10-Week wild-type (WT) and miR-7 deficiency mice with DSS treatment, and mRNA profiles of miR-7 inhibitor/NC-transfected-FHC cells and Erlotinib plus miR-7 inhibitor-transfected-FHC cells with LPS treatment,
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| Contributor(s) |
Zhao J, Xu L |
| Citation(s) |
37094666 |
| |
| Submission date |
May 12, 2021 |
| Last update date |
Mar 13, 2024 |
| Contact name |
Juan Juan Zhao |
| E-mail(s) |
jj.z.2008@163.com
|
| Phone |
13525098226
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| Organization name |
Zunyi medical University
|
| Department |
Immunology
|
| Street address |
6 Xuefu west Road
|
| City |
Zunyi |
| State/province |
Guizhou |
| ZIP/Postal code |
563000 |
| Country |
China |
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| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA729469 |
| SRA |
SRP319482 |
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