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Series GSE17453 Query DataSets for GSE17453
Status Public on Aug 05, 2009
Title Regulatory divergence in Drosophila melanogaster and D. simulans: a genome-wide analysis of allele-specific expression
Platform organism Drosophila melanogaster
Sample organisms Drosophila melanogaster; Drosophila simulans; Drosophila sp. (in: flies)
Experiment type Expression profiling by genome tiling array
Summary Species-specific regulation of gene expression contributes to the development and maintenance of reproductive isolation and to species differences in ecologically important traits. A better understanding of the evolutionary forces which shape regulatory variation and divergence can be developed by comparing expression differences among species and interspecific hybrids. Once expression differences are identified, the underlying genetics of regulatory variation or divergence can be explored. With the goal of associating cis and/or trans components of regulatory divergence with differences in gene expression, overall and allele-specific expression levels were assayed genome-wide in female adult heads of D. melanogaster, D. simulans and their F1 hybrids. A greater proportion of cis differences than trans differences were identified for genes expressed in heads and, in accordance with previous studies, cis differences also explained a larger number of species differences in overall expression level. Regulatory divergence was found to be prevalent among genes associated with defense, olfaction, and among genes downstream of the Drosophila sex determination hierarchy. In addition, two genes, with critical roles in sex determination and micro RNA processing, Sxl and loqs, were identified as misexpressed in hybrid female heads, potentially contributing to hybrid incompatibility.
Overall design Females heads from two species, D. melanogaster and D. simulans, and their F1 hybrid were compared. Genomic DNA was used as a control.
Contributor(s) Graze RM, McIntyre LM, Main B, Wayne ML, Nuzhdin SV
Citation(s) 19740431, 19667135
Submission date Jul 31, 2009
Last update date Sep 30, 2015
Contact name Rob J Kulathinal
Organization name University of Florida
Department Department of Biology
Lab McIntyre Lab
Street address 1376 Mowry Road
City Gainesville
State/province FL
ZIP/Postal code 32610
Country USA
Platforms (1)
GPL5919 [Dm35b_MR] Affymetrix Drosophila Tiling 1.0R Array
Samples (24)
GSM435267 dmel_female_heads_RNA_rep1
GSM435268 dsim_w501_female_heads_RNA_rep1
GSM435269 dsim_c1674_female_heads_RNA_rep1
BioProject PRJNA118723

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17453_RAW.tar 1000.5 Mb (http)(custom) TAR (of BAR, CEL)
Processed data provided as supplementary file

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