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Series GSE174573 Query DataSets for GSE174573
Status Public on Jan 20, 2022
Title Two novel proteins with opposing m6A writing functions interact with HAKAI [meRIP-seq]
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Using in vivo interaction proteomics, two HAKAI-interacting zinc finger proteins, HIZ1 and HIZ2, were discovered as novel components of the Arabidopsis m6A writer complex. HAKAI is required for the interaction between HIZ1 and MTA. Whilst HIZ1 knockout plants have normal levels of m6A, plants in which it is overexpressed show reduced methylation. In addition, HIZ1 was found to be involved in root hair development upon auxin transport inhibition in a HAKAI-dependent manner. Mutant plants lacking HIZ2 are viable but have an 85% reduction in m6A abundance and show severe developmental defects. Our findings suggest that HIZ1 appears to be a HAKAI-dependent negative regulator of m6A deposition and HIZ2 is a novel and essential member of the Arabidopsis m6A writer complex.
 
Overall design MeRIPSeq analysis of Arabidopsis m6A writer complex member mutants including HAKAI, Virilizer and FIP37. We used 3 replicates of each mutants and wt. Each experiment had an input and an IP sample
 
Contributor(s) Zhang M, Bodi Z, Rataj K, Zhong S, Mongan NP, Simpson GG, Fray RG
Citation(s) 35236848
Submission date May 17, 2021
Last update date Apr 21, 2022
Contact name Nigel P Mongan
Organization name University of Nottingham
Department Biodiscovery Institute
Street address University Park
City Nottingham
ZIP/Postal code NG7 2RD
Country United Kingdom
 
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (24)
GSM5319963 wt_4 input
GSM5319964 wt_4 IP
GSM5319965 wt_5 input
This SubSeries is part of SuperSeries:
GSE188611 Two zinc finger proteins with functions in m6A writing interact with HAKAI
Relations
BioProject PRJNA730562
SRA SRP320161

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Supplementary file Size Download File type/resource
GSE174573_RAW.tar 2.8 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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