 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 29, 2010 |
| Title |
Transcriptional profile of Escherichia coli K12 strain JM109 harboring pMG1 and pMG1-IrrE under 1M NaCl shock |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by array
|
| Summary |
IrrE is a unique gene in Deinococcus, which is the switch of DNA repair and celluar surival network. Expressing IrrE enhanced the salt tolence in E. coli. To understand the effect of IrrE to E. coli during salt shock, we constructed the IrrE-expressing plasmid pMG1-IrrE. And pMG1 is the empty vector used as a control.
The GroESL promoter was amplified from D. radiodurans R1 genomic DNA by PCR with proper primers. The PCR product was ligated into the T-cloning site of T-vector pMD18T, generating the plasmid pMG1.
|
| |
|
| Overall design |
RNA extracted from cells of E. coli K-12 JM109 cells with pMG1 after 4 h of growth to OD 600 achieve 0.4, or cells with pMG1 after 1mol/L NaCl shock for 10 min (and 60 min) when their OD600 achieved 0.4. RNA extracted from cells of E. coli K-12 JM109 cells with pMG1-IrrE after 4 h of growth to OD 600 achieve 0.4, or cells with pMG1-IrrE after 1mol/L NaCl shock for 10 min (and 60 min)when their OD600 achieved 0.4. pMG1 and pMG1-IrrE datasets normalized separately.
|
| |
|
| Contributor(s) |
Lin M, Pan J, Chen M, Yan Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 31, 2009 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Ming Chen |
| E-mail(s) |
chenmingbio@hotmail.com
|
| Organization name |
Biotechnology Research Institute, Chinese academy of Agricultural Sciences
|
| Street address |
Zhongguancun Nandajie 12th,Haidian Zone
|
| City |
Beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL3154 |
[E_coli_2] Affymetrix E. coli Genome 2.0 Array |
|
| Samples (18)
|
| GSM435775 |
pMG1 10 min, biological rep1 |
| GSM435776 |
pMG1 10 min, biological rep2 |
| GSM435777 |
pMG1 10 min, biological rep3 |
| GSM435778 |
pMG1 60 min, biological rep1 |
| GSM435779 |
pMG1 60 min, biological rep2 |
| GSM435780 |
pMG1 60 min, biological rep3 |
| GSM435781 |
pMG1_IrrE 0 min, biological rep1 |
| GSM435782 |
pMG1_IrrE 0 min, biological rep2 |
| GSM435783 |
pMG1_IrrE 0 min, biological rep3 |
| GSM435784 |
pMG1_IrrE 10 min, biological rep1 |
| GSM435785 |
pMG1_IrrE 10 min, biological rep2 |
| GSM435786 |
pMG1_IrrE 10 min, biological rep3 |
| GSM435787 |
pMG1_IrrE 60 min, biological rep1 |
| GSM435788 |
pMG1_IrrE 60 min, biological rep2 |
| GSM435789 |
pMG1_IrrE 60 min, biological rep3 |
|
| Relations |
| BioProject |
PRJNA118745 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE17465_RAW.tar |
15.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...