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Series GSE174780 Query DataSets for GSE174780
Status Public on May 20, 2024
Title Sperm transcriptional state associated with paternal transmission of stress phenotypes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Paternal stress can induce long-lasting changes in germ cells potentially propagating heritable changes across generations. To date, no studies have investigated differences in transmission patterns between stress-resilient and -susceptible mice. We tested the hypothesis that transcriptional alterations in sperm during chronic social defeat stress (CSDS) transmit increased susceptibility to stress phenotypes to the next generation. We demonstrate differences in offspring from stressed fathers that depend upon paternal category (resilient vs susceptible) and offspring sex. Importantly, artificial insemination reveals that sperm mediates some of the behavioral phenotypes seen in offspring. Using RNA-sequencing we report substantial and distinct changes in the transcriptomic profiles of sperm following CSDS in susceptible vs resilient fathers, with alterations in long noncoding RNAs (lncRNAs) predominating especially in susceptibility. Correlation analysis revealed that these alterations were accompanied by a loss of regulation of protein-coding genes by lncRNAs in sperm of susceptible males. We also identify several co-expression gene modules that are enriched in differentially expressed genes in sperm from either resilient or susceptible fathers. Taken together, these studies advance our understanding of intergenerational epigenetic transmission of behavioral experience.
Overall design Control vs Defeat mice sperm sequenced
Contributor(s) Cunningham AM, Walker DM, Shen L, Nestler EJ
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Submission date May 20, 2021
Last update date May 20, 2024
Contact name Aarthi Ramakrishnan
Organization name Icahn School of Medicine at Mount Sinai
Department Neuroscience
Street address 1425 Madison Avenue
City New York
State/province New York
ZIP/Postal code 10029
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (58)
GSM5328031 AMC-100-post
GSM5328032 AMC-11-post
GSM5328033 AMC-11-pre
BioProject PRJNA731510
SRA SRP320711

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE174780_RAW.tar 48.4 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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