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Status |
Public on Jan 14, 2022 |
Title |
Sustained down-regulation of alphaSmooth muscle actin expression in aortas induced by angiotensin II: a new model of vascular memory to hypertension |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We developped a new model of vascular injury memory in response to AngII stimulation in mice. We used RNAseq (aortas) to try to understand observed sustained phentoype and identify Acta2 (aSMA) as key in observed phenomenon.
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Overall design |
11-week male C57B6 mice were implanted with osmotic minipumps delivering of 2.8mg/kg/day of AngII for 2 weeks. Mice were sacrificed at the end of the treatment (AngII); and after 2 and 3 weeks of AngII (AngII+2W; Ang+3W, respectively). Age matched littermate served as control. Aortic mRNA profile was compared: CTRL vs AngII; CTRL vs AngII+2W; and CTRL vs AngII+3W
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Contributor(s) |
Pothen L, Balligand JL, Bearzatto B, Ambroise J |
Citation(s) |
35360022 |
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Submission date |
May 26, 2021 |
Last update date |
Apr 07, 2022 |
Contact name |
Jérôme Ambroise |
E-mail(s) |
jambroise83@gmail.com
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Organization name |
Université catholique de Louvain
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Department |
Institut de Recherche Expérimentale et Clinique
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Street address |
Avenue Hippocrate 54
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City |
Woluwé |
ZIP/Postal code |
1200 |
Country |
Belgium |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (8)
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Relations |
BioProject |
PRJNA732945 |
SRA |
SRP321500 |