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Series GSE176080 Query DataSets for GSE176080
Status Public on Oct 24, 2021
Title Visualizing in deceased COVID-19 patients how SARS-CoV-2 attacks the respiratory and olfactory mucosae but spares the olfactory bulb
Organisms Homo sapiens; Severe acute respiratory syndrome coronavirus 2
Experiment type Other
Expression profiling by high throughput sequencing
Summary Anosmia, the loss of smell, is a common and often the sole symptom of COVID-19. The onset of the sequence of pathobiological events leading to olfactory dysfunction remains obscure. Here, we have developed a postmortem bedside surgical procedure to harvest endoscopically samples of respiratory and olfactory mucosae and whole olfactory bulbs. Our cohort of 85 cases included COVID-19 patients who died a few days after infection with SARS-CoV-2, enabling us to catch the virus while it was still replicating. We found that sustentacular cells are the major target cell type in the olfactory mucosa. We failed to find evidence for infection of olfactory sensory neurons, and the parenchyma of the olfactory bulb is spared as well. Thus, SARS-CoV-2 does not appear to be a neurotropic virus. We postulate that transient insufficient support from sustentacular cells triggers transient olfactory dysfunction in COVID-19. Olfactory sensory neurons would become affected without getting infected.
 
Overall design One sample, olfactory mucosa of COVID case #8 containing six serial 6 µm cryosections.
 
Contributor(s) Khan M, Yoo S, Clijsters M, Backaert W, Vanstapel A, Speleman K, Lietaer C, Choi S, Dereli A, Hether TD, Marcelis L, Pan L, Reeves JW, Van Bulck P, Zhou H, Bourgeois M, Debaveye Y, De Munter P, Gunst J, Jorissen M, Lagrou K, Lorent N, Neyrinck A, Peetermans M, Rudolf Thal D, Vandenbriele C, Wauters J, Mombaerts P, Van Gerven L
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Submission date Jun 03, 2021
Last update date Oct 26, 2021
Contact name Tyler Hether
E-mail(s) thether@nanostring.com
Organization name NanoString Technologies, Inc.
Department Translational Sciences
Street address 530 Fairview Ave N
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platforms (1)
GPL29320 Illumina NovaSeq 6000 (Homo sapiens; Severe acute respiratory syndrome coronavirus 2)
Samples (17)
GSM5354566 Covid01 050520 ROI 001 well A02
GSM5354567 Covid01 050520 ROI 002 well A03
GSM5354568 Covid01 050520 ROI 003 well A04
Relations
BioProject PRJNA734853
SRA SRP322430

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE176080_Hs_R_NGS_WTA_COVID19_v1.0.pkc.gz 1.9 Mb (ftp)(http) PKC
GSE176080_RAW.tar 1.1 Mb (http)(custom) TAR (of DCC)
GSE176080_analyzed_counts.txt.gz 533.8 Kb (ftp)(http) TXT
GSE176080_collapsed_target_counts.txt.gz 398.7 Kb (ftp)(http) TXT
GSE176080_raw_counts.txt.gz 590.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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