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Status |
Public on Oct 24, 2021 |
Title |
Visualizing in deceased COVID-19 patients how SARS-CoV-2 attacks the respiratory and olfactory mucosae but spares the olfactory bulb |
Organisms |
Homo sapiens; Severe acute respiratory syndrome coronavirus 2 |
Experiment type |
Other Expression profiling by high throughput sequencing
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Summary |
Anosmia, the loss of smell, is a common and often the sole symptom of COVID-19. The onset of the sequence of pathobiological events leading to olfactory dysfunction remains obscure. Here, we have developed a postmortem bedside surgical procedure to harvest endoscopically samples of respiratory and olfactory mucosae and whole olfactory bulbs. Our cohort of 85 cases included COVID-19 patients who died a few days after infection with SARS-CoV-2, enabling us to catch the virus while it was still replicating. We found that sustentacular cells are the major target cell type in the olfactory mucosa. We failed to find evidence for infection of olfactory sensory neurons, and the parenchyma of the olfactory bulb is spared as well. Thus, SARS-CoV-2 does not appear to be a neurotropic virus. We postulate that transient insufficient support from sustentacular cells triggers transient olfactory dysfunction in COVID-19. Olfactory sensory neurons would become affected without getting infected.
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Overall design |
One sample, olfactory mucosa of COVID case #8 containing six serial 6 µm cryosections.
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Contributor(s) |
Khan M, Yoo S, Clijsters M, Backaert W, Vanstapel A, Speleman K, Lietaer C, Choi S, Dereli A, Hether TD, Marcelis L, Pan L, Reeves JW, Van Bulck P, Zhou H, Bourgeois M, Debaveye Y, De Munter P, Gunst J, Jorissen M, Lagrou K, Lorent N, Neyrinck A, Peetermans M, Rudolf Thal D, Vandenbriele C, Wauters J, Mombaerts P, Van Gerven L |
Citation missing |
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Submission date |
Jun 03, 2021 |
Last update date |
Oct 26, 2021 |
Contact name |
Tyler Hether |
E-mail(s) |
thether@nanostring.com
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Organization name |
NanoString Technologies, Inc.
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Department |
Translational Sciences
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Street address |
530 Fairview Ave N
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City |
Seattle |
State/province |
WA |
ZIP/Postal code |
98109 |
Country |
USA |
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Platforms (1) |
GPL29320 |
Illumina NovaSeq 6000 (Homo sapiens; Severe acute respiratory syndrome coronavirus 2) |
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Samples (17)
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Relations |
BioProject |
PRJNA734853 |
SRA |
SRP322430 |
Supplementary file |
Size |
Download |
File type/resource |
GSE176080_Hs_R_NGS_WTA_COVID19_v1.0.pkc.gz |
1.9 Mb |
(ftp)(http) |
PKC |
GSE176080_RAW.tar |
1.1 Mb |
(http)(custom) |
TAR (of DCC) |
GSE176080_analyzed_counts.txt.gz |
533.8 Kb |
(ftp)(http) |
TXT |
GSE176080_collapsed_target_counts.txt.gz |
398.7 Kb |
(ftp)(http) |
TXT |
GSE176080_raw_counts.txt.gz |
590.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
Processed data are available on Series record |
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