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| Status |
Public on Sep 18, 2009 |
| Title |
Gene expression in response to AGL15 during somatic embryogenesis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcript accumulation was measured using the Affymetrix Arabidopsis ATH1 Genome Array [ATH1-121501] to document changes in response to the MADS-domain transcription factor AGAMOUS-Like 15 during somatic embryogenesis.
A somatic embryo system was used where mature seed is allowed to complete germination in liquid MS media containing 2,4-D and seedlings produce somatic embryos from the shoot apical meristem (SAM) region. The frequency with which these embryos are produced directly correlates with AGL15 accumulation.
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| Overall design |
Wild type Arabidopsis (Columbia ecotype) with normal amounts of AGL15 were compared to (1) 35S:AGL15 that constitutively expresses AGL15 and produces more somatic embryos from the shoot apical region than wild type and (2) agl15 agl18 double loss-of-function mutants that produce less somatic embryos from the shoot apical region than wild type.
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| Contributor(s) |
Zheng Y, Perry SE |
| Citation(s) |
19767455, 33924312 |
| |
| Submission date |
Aug 12, 2009 |
| Last update date |
May 06, 2021 |
| Contact name |
Sharyn E Perry |
| E-mail(s) |
sperr2@uky.edu
|
| Phone |
859-218-0732
|
| Organization name |
University of Kentucky
|
| Department |
Plant and Soil Sciences
|
| Street address |
1405 Veterans Dr.
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40546-0312 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (9)
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| GSM439770 |
SAM SE 35S:AGL15, biological rep1 |
| GSM439771 |
SAM SE 35S:AGL15, biological rep2 |
| GSM439772 |
SAM SE 35S:AGL15, biological rep3 |
| GSM439773 |
SAM SE agl15 agl18 double mutant, biological rep1 |
| GSM439774 |
SAM SE agl15 agl18 double mutant, biological rep2 |
| GSM439775 |
SAM SE agl15 agl18 double mutantL, biological rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE17742 |
Global identification of targets of the Arabidopsis MADS-domain protein AGAMOUS-Like 15 |
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| Relations |
| BioProject |
PRJNA123365 |
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