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| Status |
Public on Dec 22, 2021 |
| Title |
RNA-seq profiling of foetal-derived alveolar macrophages from wildtype and Suz12 knockout mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Macrophages are at the forefront of immune responses, and their transcriptional programs are modified by their tissue environment and in response to immunological challenge. Post-translational modifications of histones, such as histone H3 lysine 27 tri-methylation (H3K27me3) by the polycomb repressive complex 2 (PRC2), are tightly associated with epigenetic regulation of gene expression. To explore whether H3K27me3 is involved in either the establishment or function of the mononuclear phagocyte system, we selectively deleted the SUZ12 gene in one-week old mice, a core component of PRC2. SUZ12 ablation induced a rapid loss of the alveolar macrophage networks under both steady state and inflammatory conditions. Ablation of SUZ12 abrogated the proliferation of these cells required for their self-renewal.
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| Overall design |
TruSeq RNA libraries were generated from FACS purified alveolar macrophages from one week old wild type (4 biological replicates) or Suz12cKO (5 biological replicates) lung. Samples were sequenced with an Illumina NextSeq 500 sequencing system, producing between 19-56 million single-end 85 bp reads per sample.
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| Contributor(s) |
Baldoni PL, Smyth GK |
| Citation(s) |
34533976 |
| |
| Submission date |
Jun 03, 2021 |
| Last update date |
Jan 13, 2022 |
| Contact name |
Gordon K Smyth |
| E-mail(s) |
smyth@wehi.edu.au
|
| Phone |
(+61 3) 9345 2326
|
| Fax |
(+61 3) 9347 0852
|
| URL |
http://www.wehi.edu.au
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
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| Department |
Bioinformatics
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| Lab |
Smyth
|
| Street address |
1G Royal Pde
|
| City |
Parkville |
| State/province |
Vic |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE176117 |
RNA-seq profiling of bone marrow-derived alveolar macrophages, foetal-derived alveolar macrophages and foetal-derived Langerhans cells from the lungs of wildtype and Suz12 knockout mice |
|
| Relations |
| BioProject |
PRJNA735045 |
| SRA |
SRP322809 |
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