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Series GSE176115 Query DataSets for GSE176115
Status Public on Dec 22, 2021
Title RNA-seq profiling of foetal-derived alveolar macrophages from wildtype and Suz12 knockout mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Macrophages are at the forefront of immune responses, and their transcriptional programs are modified by their tissue environment and in response to immunological challenge. Post-translational modifications of histones, such as histone H3 lysine 27 tri-methylation (H3K27me3) by the polycomb repressive complex 2 (PRC2), are tightly associated with epigenetic regulation of gene expression. To explore whether H3K27me3 is involved in either the establishment or function of the mononuclear phagocyte system, we selectively deleted the SUZ12 gene in one-week old mice, a core component of PRC2. SUZ12 ablation induced a rapid loss of the alveolar macrophage networks under both steady state and inflammatory conditions. Ablation of SUZ12 abrogated the proliferation of these cells required for their self-renewal.
Overall design TruSeq RNA libraries were generated from FACS purified alveolar macrophages from one week old wild type (4 biological replicates) or Suz12cKO (5 biological replicates) lung. Samples were sequenced with an Illumina NextSeq 500 sequencing system, producing between 19-56 million single-end 85 bp reads per sample.
Contributor(s) Baldoni PL, Smyth GK
Citation(s) 34533976
Submission date Jun 03, 2021
Last update date Jan 13, 2022
Contact name Gordon K Smyth
Phone (+61 3) 9345 2326
Fax (+61 3) 9347 0852
Organization name Walter and Eliza Hall Institute of Medical Research
Department Bioinformatics
Lab Smyth
Street address 1G Royal Pde
City Parkville
State/province Vic
ZIP/Postal code 3052
Country Australia
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (9)
GSM5356161 suz12 KO1
GSM5356162 suz12 KO2
GSM5356163 suz12 KO3
This SubSeries is part of SuperSeries:
GSE176117 RNA-seq profiling of bone marrow-derived alveolar macrophages, foetal-derived alveolar macrophages and foetal-derived Langerhans cells from the lungs of wildtype and Suz12 knockout mice
BioProject PRJNA735045
SRA SRP322809

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Supplementary file Size Download File type/resource
GSE176115_GenewiseCounts.tsv.gz 401.5 Kb (ftp)(http) TSV
GSE176115_RAW.tar 1.5 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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