NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE176116 Query DataSets for GSE176116
Status Public on Dec 22, 2021
Title RNA-seq profiling of foetal-derived Langerhans cells from wildtype and Suz12 knockout mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Macrophages are at the forefront of immune responses and their transcriptional programs are modified by their tissue environment and in response to immunological challenge. Post-translational modifications of histones, such as histone H3 lysine 27 tri-methylation (H3K27me3) by the polycomb repressive complex 2 (PRC2), are tightly associated with epigenetic regulation of gene expression. To explore whether H3K27me3 is involved in either the establishment or function of the mononuclear phagocyte system, we selectively deleted the SUZ12 gene in one-week old mice, a core component of PRC2.
 
Overall design SMART-Seq RNA libraries were generated from 5000 purified Langerhans cells from the skin of one week old wild mice (2 biological replicates) or Suz12cKO (3 biological replicates). Samples were sequenced with an Illumina NextSeq 500 sequencing system, producing between 22-34 million paired-end 75 bp reads per sample.
 
Contributor(s) Baldoni PL, Smyth GK
Citation(s) 34533976
Submission date Jun 03, 2021
Last update date Jan 13, 2022
Contact name Gordon K Smyth
E-mail(s) smyth@wehi.edu.au
Phone (+61 3) 9345 2326
Fax (+61 3) 9347 0852
URL http://www.wehi.edu.au
Organization name Walter and Eliza Hall Institute of Medical Research
Department Bioinformatics
Lab Smyth
Street address 1G Royal Pde
City Parkville
State/province Vic
ZIP/Postal code 3052
Country Australia
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (5)
GSM5356170 Suz12-ko-Langerhans-cell-3
GSM5356171 Suz12-ko-LC-1
GSM5356172 Suz12-ko-LC-2
This SubSeries is part of SuperSeries:
GSE176117 RNA-seq profiling of bone marrow-derived alveolar macrophages, foetal-derived alveolar macrophages and foetal-derived Langerhans cells from the lungs of wildtype and Suz12 knockout mice
Relations
BioProject PRJNA735044
SRA SRP322810

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE176116_GenewiseCounts.tsv.gz 251.7 Kb (ftp)(http) TSV
GSE176116_RAW.tar 830.0 Kb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap