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Series GSE17627 Query DataSets for GSE17627
Status Public on Aug 31, 2009
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary Little is known about the contribution of translational control to circadian rhythms. To address this issue and in particular translational control by microRNAs (miRNAs), we knocked down the miRNA biogenesis pathway in Drosophila circadian tissues. In combination with an increase in circadian-mediated transcription, this severely affected Drosophila behavioral rhythms, indicating that miRNAs function in circadian timekeeping. To identify miRNA–mRNA pairs important for this regulation, immunoprecipitation of AGO1 followed by microarray analysis identified mRNAs under miRNA-mediated control. They included three core clock mRNAs—clock (clk), vrille (vri), and clockworkorange (cwo). To identify miRNAs involved in circadian timekeeping, we exploited circadian cell-specific inhibition of the miRNA biogenesis pathway followed by tiling array analysis. This approach identified miRNAs expressed in fly head circadian tissue. Behavioral and molecular experiments show that one of these miRNAs, the developmental regulator bantam, has a role in the core circadian pacemaker. S2 cell biochemical experiments indicate that bantam regulates the translation of clk through an association with three target sites located within the clk 39 untranslated region (UTR). Moreover, clk transgenes harboring mutated bantam sites in their 39 UTRs rescue rhythms of clk mutant flies much less well than wild-type CLK transgenes.
Overall design Ago IP immunoprecipitation was performed from fly heads collected at different circadian timpoints. For wild type samples 2 biological replicates were collected for each of the six analyzed timepoints from the head protein extract (INPUT) and AGO 1-immunoprecipitated samples (IP). Gene expression was analyzed from both samples by the use of oligonucleotide microarrays. The INPUT sample corresponding to ZT3 has only one replica.
Contributor(s) Kadener S, Sugino K
Citation(s) 19696147
Submission date Aug 13, 2009
Last update date Aug 28, 2018
Contact name Ken Sugino
Organization name Janelia Research Campus
Lab NeuroSeq
Street address 19700 Helix Dr
City Ashburn
State/province VA
ZIP/Postal code 20147
Country USA
Platforms (1)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
Samples (27)
GSM440085 INPUT ZT3, replica 1
GSM440086 INPUT ZT7, replica 1
GSM440087 INPUT ZT7, replica 2
This SubSeries is part of SuperSeries:
GSE17629 Circadian analysis of miRNAs and their targets
BioProject PRJNA123389

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17627_RAW.tar 49.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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