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Series GSE17729 Query DataSets for GSE17729
Status Public on Aug 20, 2009
Title Long range spreading of dosage compensation in Drosophila captures transcribed autosomal genes inserted on X
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Dosage compensation in D. melanogaster males is achieved via targeting of the MSL complex to X chromosomal genes. This is proposed to involve initial sequence-specific recognition of the X at ~150-300 chromatin entry sites, and subsequent spreading to nearby active genes. Here we test a model in which the spreading step requires transcription and is sequence-independent. We ask whether, in the native context of the X chromosome, MSL complex will target genes of autosomal origin. We find that MSL complex does bind such genes, but only if transcriptionally active. Targeting is accompanied by acetylation of the histone H4K16 residue and two-fold transcriptional up-regulation. We conclude that the presence of a long-sought specific DNA sequence within X-linked genes is not obligatory for MSL complex binding. Instead, physical linkage and transcription play the pivotal roles in the identification of MSL targets irrespective of their origin and DNA sequence.

Keywords: Epigenetics
Overall design ChIP-seq measurements of MSL complex binding and input control. Chromatin was prepared from third instar male larvae of a genotype y w TrojanElephant; MSL3-TAP; msl3. TrojanElephant is a mini-white- and yellow-marked transposition of genomic region spanning 65 kb from cg13773 to snRNP70K. MSL3-TAP is a mini-white-marked TAP-tagged genomic msl3 transgene. IgG beads were used for pull-down.
Contributor(s) Gorchakov AA, Alekseyenko AA, Kharchenko PV, Park PJ, Kuroda MI
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Submission date Aug 19, 2009
Last update date May 15, 2019
Contact name Peter Kharchenko
Organization name Harvard Medical School
Department DBMI
Lab Kharchenko
Street address 10 Shattuck St.
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL9058 Illumina Genome Analyzer (Drosophila melanogaster)
Samples (2)
GSM442605 MSL3-TAP ChIP-seq
GSM442606 input
SRA SRP001267
BioProject PRJNA118377

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17729_RAW.tar 9.7 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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