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| Status |
Public on Sep 15, 2015 |
| Title |
Genome analysis of C. jejuni and C. coli isolated from different regions of the same chicken |
| Platform organisms |
Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; Campylobacter jejuni RM1221; Campylobacter coli RM2228 |
| Sample organisms |
Campylobacter coli; Campylobacter jejuni; Campylobacter jejuni RM1221 |
| Experiment type |
Genome variation profiling by array
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| Summary |
The gut of chicken is mostly colonised with Campylobacter jejuni and with 100 fold less C. coli. The competitive ability of C. coli OR12 over C. jejuni OR1 has been examined in experimental broiler chickens following the observation that C. coli replaced an established C. jejuni intestinal colonisation within commercial chicken flocks reared outdoors (El-Shibiny, A., Connerton, P.L., Connerton, I.F., 2005. Enumeration and diversity of campylobacters and bacteriophages isolated during the rearing cycles of free-range and organic chickens. Applied Environmental Microbiology. 71, 1259-1266).
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| Overall design |
Five independent DNA preps of C. jejuni RM1221 were labelled with Cy 5 independently and they were mixed well which was used as the control. OR1 and OR12 were labelled with Cy 3 independently and equal concentration of the control and sample DNA were used for hybridisation. Three biological replicates were done for each slide.
The supplementary file (linked at the foot of this record) represents the averaged normalised values for each experimental condition (3replicates/experimental condition).
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| Contributor(s) |
John A, El-Shibiny A, Timms A, Connerton P, Connerton I |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Aug 25, 2009 |
| Last update date |
Sep 15, 2015 |
| Contact name |
Amy John |
| E-mail(s) |
asj918@gmail.com
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| Organization name |
University of Nottingham
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| Street address |
Loughborough
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| City |
Leicestershire |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL9022 |
UON_Campylobacter oligo array v1 |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA118321 |
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