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Series GSE178401 Query DataSets for GSE178401
Status Public on Jun 17, 2024
Title LncRNA HIF1A-AS1 regulates the apoptosis and proliferation of vascular endothelial cells through globally regulating miRNAs expression [RNA-Seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary In the current study, on the basis of establishment of PA-induced damage model of HUVECs, the silencing and overexpression (OE) of HIF1A-AS1 were implemented in the HUVECs, and the effects on cell viability, apoptosis, migration and invasion were explored, respectively. Functionally, the results indicated silencing of HIF1A-AS1 promoted the proliferation migration and invasion, and reduced the apoptosis of HUVECs. In contrast, OE of HIF1A-AS1 reduced proliferation transwell and invasion, promoted the apoptosis, suggesting that HIF1A-AS1 play vital roles in regulating HUVECs. Moreover, miRNA sequencing (miRNA-seq) and RNA sequencing (RNA-seq) were carried out, and the results indicated that HIF1A-AS1 globally mediate expression of miRNA and mRNA. The multiple target genes of DEmiRNA were associated with cell proliferation and apoptosis, and overlapped with the DEGs from RNA-seq, suggestion that HIF1A-AS1 might regulate the proliferation and apoptosis of HUVECs by regulating miRNA expression.
Overall design Examination the silencing and overexpression of HIF1A-AS1 in HUVECs
Contributor(s) Chen L
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Submission date Jun 17, 2021
Last update date Jun 17, 2024
Contact name Guosong Jiang
Organization name Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
Department Department of Urology
Street address Jiefang Road, No. 1277
City WuHan
State/province Hubei Province
ZIP/Postal code 430022
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (12)
GSM5390281 HUVECs-1
GSM5390282 HUVECs-2
GSM5390283 HUVECs-3
This SubSeries is part of SuperSeries:
GSE178402 LncRNA HIF1A-AS1 regulates the apoptosis and proliferation of vascular endothelial cells through globally regulating miRNAs expression
BioProject PRJNA738885
SRA SRP324500

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Supplementary file Size Download File type/resource
GSE178401_anno_genes.FPKM.txt.gz 2.9 Mb (ftp)(http) TXT
GSE178401_genes.readcount.txt.gz 1.0 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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