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Series GSE178401 Query DataSets for GSE178401
Status Public on Jun 17, 2024
Title LncRNA HIF1A-AS1 regulates the apoptosis and proliferation of vascular endothelial cells through globally regulating miRNAs expression [RNA-Seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary In the current study, on the basis of establishment of PA-induced damage model of HUVECs, the silencing and overexpression (OE) of HIF1A-AS1 were implemented in the HUVECs, and the effects on cell viability, apoptosis, migration and invasion were explored, respectively. Functionally, the results indicated silencing of HIF1A-AS1 promoted the proliferation migration and invasion, and reduced the apoptosis of HUVECs. In contrast, OE of HIF1A-AS1 reduced proliferation transwell and invasion, promoted the apoptosis, suggesting that HIF1A-AS1 play vital roles in regulating HUVECs. Moreover, miRNA sequencing (miRNA-seq) and RNA sequencing (RNA-seq) were carried out, and the results indicated that HIF1A-AS1 globally mediate expression of miRNA and mRNA. The multiple target genes of DEmiRNA were associated with cell proliferation and apoptosis, and overlapped with the DEGs from RNA-seq, suggestion that HIF1A-AS1 might regulate the proliferation and apoptosis of HUVECs by regulating miRNA expression.
 
Overall design Examination the silencing and overexpression of HIF1A-AS1 in HUVECs
 
Contributor(s) Chen L
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jun 17, 2021
Last update date Jun 17, 2024
Contact name Guosong Jiang
E-mail(s) jiangguosongdoc@hotmail.com
Organization name Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
Department Department of Urology
Street address Jiefang Road, No. 1277
City WuHan
State/province Hubei Province
ZIP/Postal code 430022
Country China
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (12)
GSM5390281 HUVECs-1
GSM5390282 HUVECs-2
GSM5390283 HUVECs-3
This SubSeries is part of SuperSeries:
GSE178402 LncRNA HIF1A-AS1 regulates the apoptosis and proliferation of vascular endothelial cells through globally regulating miRNAs expression
Relations
BioProject PRJNA738885
SRA SRP324500

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE178401_anno_genes.FPKM.txt.gz 2.9 Mb (ftp)(http) TXT
GSE178401_genes.readcount.txt.gz 1.0 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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