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| Status |
Public on Jan 11, 2022 |
| Title |
Glucagon-like peptide-1 suppresses sympathetic activation via peripheral chemosensory inhibition |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In order to investigate the molecular basis of carotid body chemoreceptor sensitisation in the Spontaneously Hypertensive Rat (SHR) we have sequenced the transcriptomes of bilateral carotid body samples from aged-matched, male SHR and control Wistar-Kyoto rats.
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| Overall design |
Bilateral (right and left) carotid body samples of n=6 individual SHR/NHsd and WKY/NHsd (male, 12-14 weeks old) rats were microdissected from common carotid artery bifurcations following intracardial perfusion with ice-cold PBS. Total RNA was isolated using phenol-chloroform phase seperation method and Zymo Directzol RNA Microprep kit. Average RNA Integrity Number (RIN) of samples processed for sequencing (n=24) was 7.67. Poly-A enriched sequencing libraries were constructed using TruSeq® Stranded mRNA library preparation kit (20020594, Illumina). Libraries were pooled and sequenced on Illumina NextSeq500 platform using NextSeq 500/550 High Output v2.5 (150 Cycles) Kit (20024907; Illumina) on a paired-end multiplex mode.
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| Contributor(s) |
Pauza AG, Murphy D, Paton JF |
| Citation(s) |
35100822 |
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| Submission date |
Jun 20, 2021 |
| Last update date |
Mar 15, 2022 |
| Contact name |
Audrys G Pauza |
| E-mail(s) |
ap17113@bristol.ac.uk
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| Organization name |
University of Bristol
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| Department |
Bristol Medical School (THS)
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| Lab |
Murphy Group
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| Street address |
University of Bristol, The Dorothy Hodgkin Building, Whitson Street
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| City |
Bristol |
| State/province |
Somerset |
| ZIP/Postal code |
BS1 3NY |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA739448 |
| SRA |
SRP324739 |
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