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Series GSE178583 Query DataSets for GSE178583
Status Public on Nov 04, 2021
Title Early transcriptional changes in rabies virus-infected neurons and extended effects on neuronal functions
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Street-strain rabies virus primarily replicates in central nervous system without inducing significant immune response or structural damages on neurons, but the manifested symptoms of rabies indicate inherent neuronal dysfunctions in CNS. To understand the underlying state of rabies virus-infected neurons and find probable mechanisms for the neuronal dysfunction, we performed RNA-Seq at multiple time-points. This dataset provides RNA-Seq results of wild-type and mutant rabies virus-infected neuron transcriptome, with clear differential expressions between conditions. Through comparative analysis of different time-points, we have found that the matrix protein of rabies virus plays an important role in early suppression of host gene expression and maintaining control over immune response and other processes. The signaling pathways previously known to interact with rabies virus were confirmed to be modulated in this dataset, and contribute to neuronal function-associated processes. We have verified the regulation of gene expressions that could impact neuronal functions collectively, and demonstrated in calcium imaging that indeed the oscillation of calcium trace in neurons are influenced by rabies virus infection.
 
Overall design Total mRNA profile comparison of non-infected and Tha / Th4M rabies virus-infected neurons at multiple time-points after infection
 
Contributor(s) Kim S, Legendre R, Varet H, Larrous F, Grailhe R, Bourhy H
Citation(s) 34970230
Submission date Jun 21, 2021
Last update date Jan 10, 2022
Contact name Rachel Legendre
E-mail(s) rachel.legendre@pasteur.fr
Organization name Institut Pasteur
Department Research and Resource Center for Scientific Informatics
Lab Hub of Bioinformatics and Biostatistics
Street address 28, rue du docteur Roux
City Paris
ZIP/Postal code 75724
Country France
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (34)
GSM5394024 NI_0_rep1: non-infected 0hr post-infection replicate 1
GSM5394025 NI_0_rep2: non-infected 0hr post-infection replicate 2
GSM5394026 NI_0_rep3: non-infected 0hr post-infection replicate 3
Relations
BioProject PRJNA739691
SRA SRP324859

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE178583_complete_analysis.txt.gz 11.2 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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