GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE178645 Query DataSets for GSE178645
Status Public on Oct 03, 2021
Title Transcriptional activation of auxin biosynthesis drives developmental reprogramming of differentiated cells
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Plant cells exhibit remarkable plasticity of their differentiation states, enabling regeneration of whole plants from differentiated somatic cells. How they revert cell fate and express pluripotency, however, remains unclear. Here we show that transcriptional activation of auxin biosynthesis is crucial for reprogramming differentiated leaf cells in Arabidopsis. We demonstrate that intervention of histone acetyltransferases causes severe defects in callus formation from leaf mesophyll protoplasts. Our data suggest that histone acetylation affects transcription of auxin biosynthesis genes. Auxin biosynthesis is in turn required to accomplish initial cell division through the activation of G2/M phase genes mediated by MYB DOMAIN PROTEIN 3-RELATED (MYB3Rs). We further show that AUXIN RESPONSE FACTOR 7 (ARF7)/ARF19 and INDOLE-3-ACETIC ACID INDUCIBLE 3 (IAA3)/IAA18-mediated auxin signaling pathway is responsible for the cell cycle reactivation in protoplasts. These findings provide novel mechanistic insights into how differentiated plant cells revert their fate and reinitiate the cell cycle to exert pluripotency.
Overall design Expression profiling for fresh and cultured mesophyll protoplasts from Arabidopsis leaves
Contributor(s) Sakamoto Y, Sugimoto K
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jun 22, 2021
Last update date Oct 06, 2021
Contact name Yuki Sakamoto
Phone +81 45 503 9575
Organization name RIKEN
Department CSRS
Lab Cell Function Research Team
Street address 1-7-22, Suehiro-cho
City Tsurumi-ku, Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (47)
GSM5395169 23 DAS leaf replicate 1
GSM5395170 23 DAS leaf replicate 2
GSM5395171 23 DAS leaf replicate 3
BioProject PRJNA739879
SRA SRP325022

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE178645_RAW.tar 6.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap