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Series GSE17871 Query DataSets for GSE17871
Status Public on Sep 10, 2009
Title Small RNAs in budding yeast
Organisms Saccharomyces bayanus; Saccharomyces cerevisiae; Candida albicans; Naumovozyma castellii; Vanderwaltozyma polyspora
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary RNAi, a gene-silencing pathway triggered by double-stranded RNA, is conserved in diverse eukaryotic species but has been lost in the model budding yeast, Saccharomyces cerevisiae. We report that RNAi is present in other budding-yeast species, including Saccharomyces castellii and Candida albicans. These species use noncanonical Dicer proteins to generate siRNAs, which mostly correspond to transposable elements and Y´ subtelomeric repeats. In S. castellii, RNAi mutants are viable but have excess Y´ mRNA levels. In S. cerevisiae, introducing Dicer and Argonaute of S. castellii restores RNAi, and the reconstituted pathway silences endogenous retrotransposons. These results identify a novel class of Dicer proteins, bring the tool of RNAi to the study of budding yeasts, and bring the tools of budding yeast to the study of RNAi.
Overall design Employ high-throughput sequencing of endogenous small RNAs from the budding yeasts Saccharomyces castellii, Kluyveromyces polysporus, Candida albicans, Saccharomyces cerevisiae, and Saccharomyces bayanus.
Contributor(s) Drinnenberg IA, Weinberg DE, Xie KT, Mower JP, Wolfe KH, Fink GR, Bartel DP
Citation(s) 19745116
Submission date Aug 28, 2009
Last update date May 15, 2019
Contact name David Eric Weinberg
Phone 415-514-9723
Organization name University of California, San Francisco
Lab Weinberg
Street address 600 16th Street, GH S572F
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platforms (5)
GPL9132 Illumina Genome Analyzer (Vanderwaltozyma polyspora)
GPL9133 Illumina Genome Analyzer (Candida albicans)
GPL9134 Illumina Genome Analyzer (Saccharomyces cerevisiae)
Samples (9)
GSM447740 smallRNA-S.castellii-WT
GSM447741 smallRNA-S.castellii-Ago1IP
GSM447742 smallRNA-S.castellii-Input
This SubSeries is part of SuperSeries:
GSE17872 RNAi in budding yeast
SRA SRP001412
BioProject PRJNA123555

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17871_RAW.tar 62.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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