|
| Status |
Public on Sep 10, 2009 |
| Title |
Small RNAs in budding yeast |
| Organisms |
Saccharomyces bayanus; Saccharomyces cerevisiae; Candida albicans; Naumovozyma castellii; Vanderwaltozyma polyspora |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
RNAi, a gene-silencing pathway triggered by double-stranded RNA, is conserved in diverse eukaryotic species but has been lost in the model budding yeast, Saccharomyces cerevisiae. We report that RNAi is present in other budding-yeast species, including Saccharomyces castellii and Candida albicans. These species use noncanonical Dicer proteins to generate siRNAs, which mostly correspond to transposable elements and Y´ subtelomeric repeats. In S. castellii, RNAi mutants are viable but have excess Y´ mRNA levels. In S. cerevisiae, introducing Dicer and Argonaute of S. castellii restores RNAi, and the reconstituted pathway silences endogenous retrotransposons. These results identify a novel class of Dicer proteins, bring the tool of RNAi to the study of budding yeasts, and bring the tools of budding yeast to the study of RNAi.
|
| |
|
| Overall design |
Employ high-throughput sequencing of endogenous small RNAs from the budding yeasts Saccharomyces castellii, Kluyveromyces polysporus, Candida albicans, Saccharomyces cerevisiae, and Saccharomyces bayanus.
|
| |
|
| Contributor(s) |
Drinnenberg IA, Weinberg DE, Xie KT, Mower JP, Wolfe KH, Fink GR, Bartel DP |
| Citation(s) |
19745116 |
| |
| Submission date |
Aug 28, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
David Eric Weinberg |
| E-mail(s) |
david.weinberg@ucsf.edu
|
| Phone |
415-514-9723
|
| Organization name |
University of California, San Francisco
|
| Lab |
Weinberg
|
| Street address |
600 16th Street, GH S572F
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platforms (5)
|
| GPL9132 |
Illumina Genome Analyzer (Vanderwaltozyma polyspora) |
| GPL9133 |
Illumina Genome Analyzer (Candida albicans) |
| GPL9134 |
Illumina Genome Analyzer (Saccharomyces cerevisiae) |
| GPL9135 |
Illumina Genome Analyzer (Saccharomyces bayanus) |
| GPL15027 |
Illumina Genome Analyzer (Naumovozyma castellii) |
|
| Samples (9)
|
|
| This SubSeries is part of SuperSeries: |
|
| Relations |
| SRA |
SRP001412 |
| BioProject |
PRJNA123555 |
Less...
More...