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| Status |
Public on Oct 07, 2022 |
| Title |
Prostate cancer resistance leads to a global deregulation of translation factors and unconventional translation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Using a newly generated enzalutamide-resistant prostate cancer model, we characterized alterations in the translatome during prostate cancer enzalutamide resistance acquisition. We show a discordance between the translatome and transcriptome of drug-resistant cells, a deregulation of proteins involved in translation and an overall decrease in translational efficiency upon resistance acquisition. Interestingly, long non-coding RNAs show higher translation efficiency in enzalutamide-resistant cells, and a strong correlation with poor patient prognosis. Taken together, this suggests that aberrant translation of coding and non-coding genes are strong indicators of PCa enzalutamide-resistance.
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| Overall design |
RNA sequencing of Poly(A) RNA from enzalutamide sensitive VCaP and VCaP-CRPC (Castration Resistant Prostate Cancer) and of enzalutamide resistant VCaP-ER (Enzalutamide Resistant), and RNA sequencing of heavy polysome-bound RNA from VCaP-CRPC and VCaP-ER (as duplicates).
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| |
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| Contributor(s) |
Lelong EJ, Khelifi G, Hussein SI |
| Citation(s) |
36348939 |
| |
| Submission date |
Jun 29, 2021 |
| Last update date |
Nov 21, 2022 |
| Contact name |
Samer M.I. Hussein |
| E-mail(s) |
samer.hussein@fmed.ulaval.ca
|
| Organization name |
CHU de Québec - Université Laval
|
| Department |
Departement of medecine
|
| Lab |
Hussein Lab
|
| Street address |
9 rue Mcmahon
|
| City |
Quebec City |
| State/province |
Quebec |
| ZIP/Postal code |
G1R 3S3 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (10)
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| Relations |
| BioProject |
PRJNA742424 |
| SRA |
SRP326162 |
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