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| Status |
Public on Dec 01, 2023 |
| Title |
Chromomal translocations induced by CRISPR-Cas9 persist in expanded T cells |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Chromosomal translocations cause various lymphoid cancers and are prevalent in CRISPR-Cas9-meidated gene editing. However, the fate of gene editing-induced translocations remains elusive in vivo. Here, we found that translocations in engineered T cells experience clonal expansion after transferring into recipients for three weeks and remains comparable level with edited T cell before transfer, arguing against the notion that translocations would be gradually lost during cell proliferation due to growth retardation.
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| |
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| Overall design |
Examination of the fate of chromosomal translocation induced by gene editing in T cells
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| |
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| Contributor(s) |
Liu Y, Wu J, Hu J |
| Citation missing |
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| |
| Submission date |
Jul 11, 2021 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Yang Liu |
| E-mail(s) |
liu.y@pku.edu.cn
|
| Organization name |
School of life sciences, Peking University
|
| Lab |
Jiazhi Hu lab
|
| Street address |
Yiheyuan Road, No. 5
|
| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA745461 |
| SRA |
SRP327854 |
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