|
| Status |
Public on Sep 30, 2021 |
| Title |
RNA-seq analysis of freshly isolated and ex-vivo cultured human cord blood CD34+ cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-seq analysis was performed to compare differentially expressed genes in freshly isolated and ex-vivo cultured human cord blood CD34+ cells. Mitochondrion related genes are upregulated in CD34+ hematopoietic stem and progenitor cells upon ex vivo culture. In vivo transplantation experiments demonstrate that stemness of CD34+ cells is significantly decreased due to oxidative stress induced by ex vivo culture.
|
| |
|
| Overall design |
Freshly isolated cord blood CD34+ cells (Group 0) and day-4 ex-vivo cultured cord blood CD34+ cells (Group 4) are subjected to RNA-seq analysis.
|
| |
|
| Contributor(s) |
Guo B, Broxmeyer HE, Wan J, Liu S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 12, 2021 |
| Last update date |
Sep 30, 2021 |
| Contact name |
Bin Guo |
| Organization name |
Indiana University
|
| Department |
Microbiology & Immunology
|
| Lab |
Dr. Hal Broxmeyer Lab
|
| Street address |
950 W. Walnut Street
|
| City |
Indianapolis |
| State/province |
Indiana |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA745928 |
| SRA |
SRP327956 |
Less...
More...