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Series GSE179928 Query DataSets for GSE179928
Status Public on Sep 30, 2021
Title RNA-seq analysis of freshly isolated and ex-vivo cultured human cord blood CD34+ cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary RNA-seq analysis was performed to compare differentially expressed genes in freshly isolated and ex-vivo cultured human cord blood CD34+ cells. Mitochondrion related genes are upregulated in CD34+ hematopoietic stem and progenitor cells upon ex vivo culture. In vivo transplantation experiments demonstrate that stemness of CD34+ cells is significantly decreased due to oxidative stress induced by ex vivo culture.
Overall design Freshly isolated cord blood CD34+ cells (Group 0) and day-4 ex-vivo cultured cord blood CD34+ cells (Group 4) are subjected to RNA-seq analysis.
Contributor(s) Guo B, Broxmeyer HE, Wan J, Liu S
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Submission date Jul 12, 2021
Last update date Sep 30, 2021
Contact name Bin Guo
Organization name Indiana University
Department Microbiology & Immunology
Lab Dr. Hal Broxmeyer Lab
Street address 950 W. Walnut Street
City Indianapolis
State/province Indiana
ZIP/Postal code 46202
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (8)
GSM5437066 cord blood CD34+ cell Group0-1
GSM5437067 cord blood CD34+ cell Group0-2
GSM5437068 cord blood CD34+ cell Group0-3
BioProject PRJNA745928
SRA SRP327956

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Supplementary file Size Download File type/resource
GSE179928_readCounts.xlsx 10.9 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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