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Series GSE180217 Query DataSets for GSE180217
Status Public on Jun 08, 2023
Title Germ-cell specific eIF4E1b regulates maternal mRNA translation to ensure zygotic genome activation [RIP-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Translation of maternal mRNAs is detected before transcription of zygotic genes and is essential for mammalian embryo development. How certain maternal mRNAs are selected for translation instead of degradation and how this burst of translation affects zygotic genome activation remains unknown. Using gene-edited mice, we document that the oocyte-specific eukaryotic translation initiation factor 4E family member 1b (eIF4E1b) is the regulator of maternal mRNA expression that ensures subsequent reprogramming of the zygotic genome. In oocytes, eIF4E1b binds to transcripts encoding translation machinery proteins, chromatin remodelers and reprogramming factors to promote their translation in zygotes and protect them from degradation. The protein products are thought to establish an open chromatin landscape in one-cell zygotes to enable transcription of genes required for cleavage stage development. Our results define a program for rapid resetting of the zygotic epigenome that is regulated by maternal mRNA expression and provides new insights into the mammalian maternal-to-zygotic transition.
 
Overall design RNA profiles after IP with anti-HA beads using M2 eggs or early 2-cell embryos collected from Eif4e1b FLAGHA knock-in(KI) females or WT controls.
 
Contributor(s) Yang G, Xin Q, Dean J
Citation(s) 37257918
Submission date Jul 15, 2021
Last update date Sep 07, 2023
Contact name Guanghui Yang
E-mail(s) guanghui.yang@nih.gov
Organization name National Institutes of Health
Department National Institute of Diabetes and Digestive and Kidney Diseases
Lab Laboratory of Cellular and Developmental Biology
Street address 50 South Drive
City Bethesda
State/province Maryland
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (24)
GSM5456219 M2_WT_IP_S1
GSM5456220 M2_WT_IP_S2
GSM5456221 M2_WT_IP_S3
This SubSeries is part of SuperSeries:
GSE180218 Germ cell-specific eIF4E1b regulates maternal mRNA translation to ensure zygotic genome activation
Relations
BioProject PRJNA747037
SRA SRP328593

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE180217_Eif4e1b_RIPSeq_gene_count_matrix.csv.gz 1.6 Mb (ftp)(http) CSV
GSE180217_RAW.tar 31.9 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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