|
| Status |
Public on Jun 08, 2023 |
| Title |
Germ-cell specific eIF4E1b regulates maternal mRNA translation to ensure zygotic genome activation [RIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Translation of maternal mRNAs is detected before transcription of zygotic genes and is essential for mammalian embryo development. How certain maternal mRNAs are selected for translation instead of degradation and how this burst of translation affects zygotic genome activation remains unknown. Using gene-edited mice, we document that the oocyte-specific eukaryotic translation initiation factor 4E family member 1b (eIF4E1b) is the regulator of maternal mRNA expression that ensures subsequent reprogramming of the zygotic genome. In oocytes, eIF4E1b binds to transcripts encoding translation machinery proteins, chromatin remodelers and reprogramming factors to promote their translation in zygotes and protect them from degradation. The protein products are thought to establish an open chromatin landscape in one-cell zygotes to enable transcription of genes required for cleavage stage development. Our results define a program for rapid resetting of the zygotic epigenome that is regulated by maternal mRNA expression and provides new insights into the mammalian maternal-to-zygotic transition.
|
| |
|
| Overall design |
RNA profiles after IP with anti-HA beads using M2 eggs or early 2-cell embryos collected from Eif4e1b FLAGHA knock-in(KI) females or WT controls.
|
| |
|
| Contributor(s) |
Yang G, Xin Q, Dean J |
| Citation(s) |
37257918 |
| |
| Submission date |
Jul 15, 2021 |
| Last update date |
Sep 07, 2023 |
| Contact name |
Guanghui Yang |
| E-mail(s) |
guanghui.yang@nih.gov
|
| Organization name |
National Institutes of Health
|
| Department |
National Institute of Diabetes and Digestive and Kidney Diseases
|
| Lab |
Laboratory of Cellular and Developmental Biology
|
| Street address |
50 South Drive
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (24)
|
|
| This SubSeries is part of SuperSeries: |
| GSE180218 |
Germ cell-specific eIF4E1b regulates maternal mRNA translation to ensure zygotic genome activation |
|
| Relations |
| BioProject |
PRJNA747037 |
| SRA |
SRP328593 |
Less...
More...