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| Status |
Public on Dec 31, 2022 |
| Title |
Urinary exosomal miRNA-663a shows variable expression in diabetic kidney disease patients with or without proteinuria |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
|
| Summary |
HK-2 cells were cultured in low and high glucose conditions for 96 hours in serum free conditions. Exosomes were isolated from the supernatant of these cells. miRCURY LNATM Universal RT microRNA PCR Human panel I+II (Exiqon) were used to study the differential expression from three different high glucose treated exosomes (HE1, HE2, HE3) than to low glucose treated exosomes (LE1, LE2, LE3).
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| Overall design |
qPCR miRNA expression profiling from three different exosomal samples. Each sample was isolated from low and high glucose treated HK-2 cells
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| Web link |
https://www.nature.com/articles/s41598-022-26558-4
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| |
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| Contributor(s) |
Sinha N, Puri V, Kumar V, Nada R, Rastogi A, Jha V, Puri S |
| Citation(s) |
36934129 |
| Submission date |
Jul 19, 2021 |
| Last update date |
Apr 05, 2023 |
| Contact name |
sanjeev puri |
| E-mail(s) |
s_puri@pu.ac.in
|
| Phone |
9872580078
|
| Organization name |
panjab university
|
| Department |
uiet, biotechnology branch
|
| Street address |
sector-14
|
| City |
chandigarh |
| State/province |
160014 |
| ZIP/Postal code |
160014 |
| Country |
India |
| |
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| Platforms (1) |
| GPL30410 |
miRCURY LNA™ Universal RT microRNA PCR, microRNA Ready-to-Use PCR, Human panel I and II V4 |
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| Samples (6)
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| GSM5462422 |
low glucose treated HK-2 cells derived exosomes from one batch of cells |
| GSM5462423 |
low glucose treated HK-2 cells derived exosomes from second batch of cells |
| GSM5462424 |
low glucose treated HK-2 cells derived exosomes from third batch of cells |
| GSM5462425 |
high glucose treated HK-2 cells derived exosomes from one batch of cells |
| GSM5462426 |
high glucose treated HK-2 cells derived exosomes from second batch of cells |
| GSM5462427 |
high glucose treated HK-2 cells derived exosomes from third batch of cells |
|
| Relations |
| BioProject |
PRJNA748079 |
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