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| Status |
Public on Jan 21, 2010 |
| Title |
Expression profiling in dopaminergic brain structures of rats self-administering cocaine |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene expression profiling in dopaminergic brain structures of rats self-administering cocaine. Effect of histone deacetylase inhibition
We have shown that injection of the HDAC inhibitor trichostatin A (TsA) to rats is sufficient to decrease their motivation to self-administer cocaine. The aim of the present study was to investigate alterations in gene expression patterns in the Anterior Cingulate Cortex and Nucleus Accumbens of rats self-administering cocaine and treated repeatedly with TsA, and compare them with rats taking only cocaine.
We used Affymetrix microarrays to identify genes the expression of which was up-regulated or downregulated during this process.
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| Overall design |
Drug self-administration was performed in dark operant chambers under a fixed-ratio 1 schedule of reinforcement that was carried out for 4 days during daily 2 h sessions. Each nosepoke into the active hole triggered the i.v. delivery of a 40 μl cocaine solution (0,3 mg/kg/injection) under the control of the computer. Rats were sacrificed 2 h after the 4th self-administration session; the anterior cingulate cortex and the nucleus accumbens were then dissected.
Two treatments comparison
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| Contributor(s) |
Zwiller J, Host L, Dembele D, Thibault C |
| Citation(s) |
20132486 |
| |
| Submission date |
Sep 10, 2009 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Doulaye Dembele |
| E-mail(s) |
doulaye@igbmc.fr
|
| Phone |
+33 3 88 65 35 28
|
| Organization name |
IGBMC
|
| Department |
Biopuces
|
| Street address |
1 rue Laurent Fries
|
| City |
Illkirch |
| ZIP/Postal code |
67400 |
| Country |
France |
| |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (12)
|
| GSM451395 |
Anterior Cingulate Cortex Cocaine Self-administration Vehicle treatment replicate 1 |
| GSM451396 |
Anterior Cingulate Cortex Cocaine Self-administration Vehicle treatment replicate 2 |
| GSM451397 |
Anterior Cingulate Cortex Cocaine Self-administration Vehicle treatment replicate 3 |
| GSM451398 |
Anterior Cingulate Cortex Cocaine Self-administration TsA treatment replicate 1 |
| GSM451399 |
Anterior Cingulate Cortex Cocaine Self-administration TsA treatment replicate 2 |
| GSM451400 |
Anterior Cingulate Cortex Cocaine Self-administration TsA treatment replicate 3 |
| GSM451401 |
Nucleus Accumbens Cocaine Self-adminitration Vehicle treatment replicate 1 |
| GSM451402 |
Nucleus Accumbens Cocaine Self-adminitration Vehicle treatment replicate 2 |
| GSM451403 |
Nucleus Accumbens Cocaine Self-adminitration Vehicle treatment replicate 3 |
| GSM451404 |
Nucleus Accumbens Cocaine Self-adminitration TsA treatment replicate 1 |
| GSM451405 |
Nucleus Accumbens Cocaine Self-adminitration TsA treatment replicate 2 |
| GSM451406 |
Nucleus Accumbens Cocaine Self-adminitration TsA treatment replicate 3 |
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| Relations |
| BioProject |
PRJNA119315 |
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