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| Status |
Public on Feb 26, 2024 |
| Title |
Gene regulatory basis of bystander activation in CD8+ T cells (RNA-seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The immune system is stratified into layers of specialized cells with distinct functions. Recently, Lin28b was shown to serve as a master regulator of fetal lymphopoiesis and program the development of more innate-like lymphocytes in early life. However, it is still unclear how Lin28b alters the function of fetal-derived lymphocytes and protects the host against infection. In this report, we examined how Lin28b transcriptionally and epigenetically programs murine CD8+ T cells for innate immune defense and translated our key findings to human CD8+ T cells. We found that Lin28b operates as a gatekeeper, opening the door to a hidden diversity of phenotypes that are unveiled upon stimulation with innate cytokines. In particular, Lin28b promotes a transcriptionally and functionally diverse pool of cells through chromatin remodeling. As a result, neonatal CD8+ T cells are able to deploy a bet-hedging immune strategy and protect the host against a wide range of unrelated pathogens.
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| Overall design |
Gene expression profiling of gBT-I TCR tg adult, neonatal and Lin28b Tg adult CD8+ T cells treated with IL-2 or IL-12/18 by high-throughput sequencing, in 2 or 6 replicates, using Illumina NextSeq500.
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| Contributor(s) |
Watson NB, Patel RK, Grimson AW, Rudd BD |
| Citation(s) |
38394230 |
| |
| Submission date |
Jul 23, 2021 |
| Last update date |
Feb 26, 2024 |
| Contact name |
Andrew Grimson |
| Organization name |
Cornell University
|
| Department |
Molecular Biology and Genetics
|
| Street address |
445 Biotech Bldg
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14850 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (20)
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| This SubSeries is part of SuperSeries: |
| GSE180732 |
Gene regulatory basis of bystander activation in CD8+ T cells |
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| Relations |
| BioProject |
PRJNA749293 |
| SRA |
SRP329628 |
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