NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE181425 Query DataSets for GSE181425
Status Public on Mar 01, 2022
Title Single cell RNA sequencing from stroma in the E16 salivary gland
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Use scRNA Seq to find B10stromal subpopulations and define how these subpopulations contribute to salivary gland proacinar differentiation.
Method: scRNA Seq on stromal enriched cells from E16 salivary glands. Model closely related cells using PCA and UMAP regressions. Find genes that broadly and specifically define cell subpopulations.
Results: The epithelium and stromal fibroblasts have multiple subpopulations. Stromal cells can be subdivided by their expression of Pdgfra, Pdgfrb, Thy1, Wnt2, and Acta2.
Conclusions: Pdgfra expressing stroma specifically use BMP7 to promote proacinar differentiation.
 
Overall design Transcriptome analysis of stromal cell subpopulations
 
Contributor(s) Moskwa N, Nelson DA, Forni P, Larsen M
Citation(s) 35224622, 36945483, 37287453
Submission date Aug 03, 2021
Last update date Sep 07, 2023
Contact name Nicholas Moskwa
E-mail(s) nmoskwa@albany.edu
Organization name University at Albany
Street address 1400 Washington Avenue
City Albany
State/province New York
ZIP/Postal code 12222
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (1)
GSM5502238 Embryonic Salivary Gland scRNA Sequencing
This SubSeries is part of SuperSeries:
GSE181430 Single-cell sequencing reveals PDFGRα+ stromal cell subpopulations that promote proacinar differentiation in embryonic salivary gland organoids
Relations
BioProject PRJNA751935
SRA SRP331061

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE181425_All_Gene_Averages_perCluster_E16_04.csv.gz 1.7 Mb (ftp)(http) CSV
GSE181425_All_Gene_Averages_perEpiCluster_E16_04.csv.gz 222.0 Kb (ftp)(http) CSV
GSE181425_All_Gene_Averages_perStromaCluster_E16_04.csv.gz 287.0 Kb (ftp)(http) CSV
GSE181425_RAW.tar 110.7 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap