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Status |
Public on Mar 01, 2022 |
Title |
Single cell RNA sequencing from stroma in the E16 salivary gland |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Purpose: Use scRNA Seq to find B10stromal subpopulations and define how these subpopulations contribute to salivary gland proacinar differentiation. Method: scRNA Seq on stromal enriched cells from E16 salivary glands. Model closely related cells using PCA and UMAP regressions. Find genes that broadly and specifically define cell subpopulations. Results: The epithelium and stromal fibroblasts have multiple subpopulations. Stromal cells can be subdivided by their expression of Pdgfra, Pdgfrb, Thy1, Wnt2, and Acta2. Conclusions: Pdgfra expressing stroma specifically use BMP7 to promote proacinar differentiation.
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Overall design |
Transcriptome analysis of stromal cell subpopulations
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Contributor(s) |
Moskwa N, Nelson DA, Forni P, Larsen M |
Citation(s) |
35224622, 36945483, 37287453 |
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Submission date |
Aug 03, 2021 |
Last update date |
Sep 07, 2023 |
Contact name |
Nicholas Moskwa |
E-mail(s) |
nmoskwa@albany.edu
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Organization name |
University at Albany
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Street address |
1400 Washington Avenue
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City |
Albany |
State/province |
New York |
ZIP/Postal code |
12222 |
Country |
USA |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (1) |
GSM5502238 |
Embryonic Salivary Gland scRNA Sequencing |
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This SubSeries is part of SuperSeries: |
GSE181430 |
Single-cell sequencing reveals PDFGRα+ stromal cell subpopulations that promote proacinar differentiation in embryonic salivary gland organoids |
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Relations |
BioProject |
PRJNA751935 |
SRA |
SRP331061 |