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Series GSE181555 Query DataSets for GSE181555
Status Public on Aug 08, 2021
Title Melatonin reverses mitochondria dysfunction and oxidative stress-induced apoptosis of Sudan I-exposed mouse oocytes Exposure to Sudan I compromises oocyte quality via the induction of mitochondrial dysfunction and oxidative stress in mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Sudan I is one of the industry dyes and widely used in cosmetics, wax agent, solvent and textile. Sudan I has multiple toxicity such as carcinogenicity, mutagenicity, genotoxicity and oxidative damage. However, Sudan I has been illegally used as colorant in food products, triggering worldwide attention about food safety. Nevertheless, the toxicity of Sudan I on reproduction, particularly on oocyte maturation is still unclear. In the present study, using mouse in vivo models, we report the toxicity effects of Sudan I on mouse oocyte. The results reflect that Sudan I exposure disrupts spindle organization and chromosomes alignment as well as cortical actin distribution, thus leading to the failure of polar body extrusion. Based on the transcriptome results, it is found that the exposure of Sudan I leads to the change in expression of 764 genes. Moreover, it’s further reflected that the damaging effects of Sudan I are mediated by the destruction of mitochondrial functions, which induces the accumulated ROS to stimulate oxidative stress-induced apoptosis, while in vivo supplementation of melatonin efficaciously suppresses mitochondrial dysfunction and the accompanying apoptosis, thus reverses oocyte meiotic deteriorations. Collectively, our results prove the reproduction toxicity of Sudan I for the exposure of Sudan I reduces the oocyte quality, and demonstrate a feasible solution to mitigating Sudan I-induced meiotic deteriorations.
Overall design Transcriptomic analysis of oocytes control, Sudan I-exposed, and melatonin rescue groups. was carried out using a protocol for RNA-Seq. The constructed library was sequenced with Geekgene and Gene Denovo platform.
Contributor(s) Sun S, Zhang Y
Citation(s) 34544023
Submission date Aug 06, 2021
Last update date Sep 28, 2021
Contact name 春华 邢
Phone 15033595386
Organization name 南京农业大学
Street address 江苏省南京市玄武区孝陵卫街道
City 南京
State/province 江苏
ZIP/Postal code 210000
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM5506046 Control-1
GSM5506047 Control-2
GSM5506048 Sudan-1
BioProject PRJNA752574
SRA SRP331333

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GSE181555_Process_data.xlsx 1.0 Mb (ftp)(http) XLSX
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