GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE182311 Query DataSets for GSE182311
Status Public on Jun 29, 2022
Title β-cell-specific deletion of Zfp148 improves nutrient-stimulated β-cell Ca2+ responses
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Insulin secretion from pancreatic β-cells is essential for glucose homeostasis. An insufficient response to the demand for insulin results in diabetes. We previously showed that β-cell-specific deletion of Zfp148 (β-Zfp148KO) improves glucose tolerance and insulin secretion in mice. These RNA sequencing data show pathways altered in the β-Zfp148KO consistent with altered PEP cycling and improved insulin secretion responses.
Overall design Details of generating the β-Zfp148KO and their control littermates is provided by Keller et al. JCI 2019 ( 5 female littermate control (floxed Zfp148), 6 female β-Zfp148KO, 5 male control, and 4 male β-Zfp148KO mice were placed on a high-fat, high-sucrose diet (TD.08811) for 20 weeks. They were then sacrificed. RNA extracts and cDNA were prepared from whole islets harvested from these mice. RNA sequencing was performed by the UW-Madison Biotechnology Center. Processed sequencing data were analyzed by EBSeq (for EBSeq method, see Leng et al.
Contributor(s) Emfinger CH, Attie AD, Keller MP
Citation(s) 35603790
Submission date Aug 17, 2021
Last update date Jun 29, 2022
Contact name Alan Attie
Organization name University of Wisconsin-Madison
Department Biochemistry
Lab Attie lab
Street address 433 Babcock Drive
City Madison
State/province Wisconsin
ZIP/Postal code 53706
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (20)
GSM5526497 82
GSM5526498 83
GSM5526499 91
BioProject PRJNA755728
SRA SRP332941

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE182311_Emfinger_et_al_RNA_seq_PPDE_0.95.xlsx 22.1 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap