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Series GSE182311 Query DataSets for GSE182311
Status Public on Jun 29, 2022
Title β-cell-specific deletion of Zfp148 improves nutrient-stimulated β-cell Ca2+ responses
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Insulin secretion from pancreatic β-cells is essential for glucose homeostasis. An insufficient response to the demand for insulin results in diabetes. We previously showed that β-cell-specific deletion of Zfp148 (β-Zfp148KO) improves glucose tolerance and insulin secretion in mice. These RNA sequencing data show pathways altered in the β-Zfp148KO consistent with altered PEP cycling and improved insulin secretion responses.
 
Overall design Details of generating the β-Zfp148KO and their control littermates is provided by Keller et al. JCI 2019 (https://doi.org/10.1172/JCI129143). 5 female littermate control (floxed Zfp148), 6 female β-Zfp148KO, 5 male control, and 4 male β-Zfp148KO mice were placed on a high-fat, high-sucrose diet (TD.08811) for 20 weeks. They were then sacrificed. RNA extracts and cDNA were prepared from whole islets harvested from these mice. RNA sequencing was performed by the UW-Madison Biotechnology Center. Processed sequencing data were analyzed by EBSeq (for EBSeq method, see Leng et al. https://doi.org/10.1093/bioinformatics/btt087).
 
Contributor(s) Emfinger CH, Attie AD, Keller MP
Citation(s) 35603790
Submission date Aug 17, 2021
Last update date Jun 29, 2022
Contact name Alan Attie
E-mail(s) adattie@wisc.edu, attie@biochem.wisc.edu
Organization name University of Wisconsin-Madison
Department Biochemistry
Lab Attie lab
Street address 433 Babcock Drive
City Madison
State/province Wisconsin
ZIP/Postal code 53706
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (20)
GSM5526497 82
GSM5526498 83
GSM5526499 91
Relations
BioProject PRJNA755728
SRA SRP332941

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE182311_Emfinger_et_al_RNA_seq_PPDE_0.95.xlsx 22.1 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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