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Series GSE18245 Query DataSets for GSE18245
Status Public on Sep 24, 2010
Title Elucidating the stromal expression pattern in response to tumor-derived Shh
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Most tumors of the upper gastrointestinal tract are known to depend on an excessive expression of Shh. It was recently discovered that this Shh does not signal on the tumor cells, but rather the stromal cells that in turn produce an unknown set of reciprocal signals that act as growth- or survival cues for the tumor cells. This sequencing effort aims to identify the reciprocal signals.
Overall design Mouse fibroblasts and Shh-expressing human pancreatic adenocarcinoma cells were either grown alone, or in a non-adherent coculture system. To be able to assess the effect of Shh on fibroblast gene expression (being the reciprocal signal), 5E1 blocking antibody was added to the cells and after 5 days, RNA was isolated. n=1
Contributor(s) Bijlsma MF, Roelink H
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Submission date Sep 24, 2009
Last update date May 15, 2019
Contact name henk roelink
Organization name UC Berkeley
Department MCB
Lab roelink
Street address 16 barker hall
City berkeley
State/province CA
ZIP/Postal code 94720-3204
Country USA
Platforms (3)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
GPL9185 Illumina Genome Analyzer (Mus musculus)
GPL10329 Illumina Genome Analyzer (Homo sapiens; Mus musculus)
Samples (4)
GSM455884 MFB_1_sequence
GSM455885 MFB_2_sequence
GSM455886 MFB_3_sequence
SRA SRP002306
BioProject PRJNA119635

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18245_RAW.tar 2.7 Gb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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