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Series GSE182476 Query DataSets for GSE182476
Status Public on Mar 23, 2022
Title 24-nt phasiRNAs move from tapetal to meiotic cells in maize anthers
Organism Zea mays
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary In maize, 24-nt phased, secondary small interfering RNAs (phasiRNAs) are abundant in meiotic stage anthers, but their distribution and functions are not precisely known. Using laser capture microdissection (LCM), we analyzed tapetal cells, meiocytes, and other somatic cells at several stages of anther development to establish the timing of 24-PHAS precursor transcripts and the 24-nt phasiRNA products. This dataset includes 24-nt phasiRNA part of data. 24-nt phasiRNAs are found to accumulate in all cell types, with the highest levels in meiocytes, followed by tapetum.
 
Overall design small RNA profiling of fixed single W23 inbred maize anther and isolated meiocyte, tapetum, and outer cell layers.
 
Contributor(s) Walbot V, Zhou X, Meyers BC, Teng C
Citation(s) 35451503
Submission date Aug 19, 2021
Last update date Jun 23, 2022
Contact name Blake C. Meyers
E-mail(s) bmeyers@danforthcenter.org
Phone 314-587-1422
Organization name Donald Danforth Plant Science Center
Lab Meyers lab
Street address 975 N Warson Road
City St. Louis
State/province MO
ZIP/Postal code 63132
Country USA
 
Platforms (1)
GPL27775 NextSeq 550 (Zea mays)
Samples (18)
GSM5529924 W23_1_5mm, replicate 1
GSM5529925 W23_1_5mm, replicate 2
GSM5529926 ME_1_5mm, replicate 1
Relations
BioProject PRJNA756318
SRA SRP333382

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE182476_RAW.tar 171.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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