|
| Status |
Public on Aug 27, 2021 |
| Title |
RNAseq analysis comparing siRNA knocked down FOXF2 in and MKX in human normotrophic scar dermal fibroblasts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
3mm punch biopsies were taken from a healed normotrophic scar in burn patients with a scar at least 1 year old and fibroblasts were cultured from explants. Previous transcriptomic and epigenomic work found MKX and FOXF2 genes were overexpressed and these were knocked down using siRNA. RNA was then extracted and analysed using RNAseq to determine genes and pathways affected by this knockdown
|
| |
|
| Overall design |
3 patients fibroblasts were transfected with either scrambled siRNA, MKX siRNA or FOXF2 siRNA
|
| Web link |
https://www.sciencedirect.com/science/article/pii/S0022202X21023538?via%3Dihub
|
| |
|
| Contributor(s) |
Stevenson AW, Deng Z |
| Citation(s) |
34687743 |
| |
| Submission date |
Aug 20, 2021 |
| Last update date |
Dec 15, 2023 |
| Contact name |
Andrew William Stevenson |
| E-mail(s) |
andrew.stevenson@uwa.edu.au
|
| Phone |
864887515
|
| Organization name |
University of Western Australia
|
| Department |
School of Biomedical Sciences
|
| Lab |
Burn Injury Research Unit
|
| Street address |
Room 112b, Curnow Building, Hackett Drive Carpark Entrance 2, UWA
|
| City |
Crawley |
| State/province |
Western Australia |
| ZIP/Postal code |
6009 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA756550 |
| SRA |
SRP333477 |
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