|
| Status |
Public on Aug 29, 2021 |
| Title |
Isolation and expansion of murine γδ T cells from mouse splenocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To characterise gene expression of in vitro expanded mouse gammadelta T cells.
Methods: Freshly isolated and IL-2 expanded gammadelta T cells, single samples, and total RNA was isolated and sequenced.
Results: High upregulation of Th1 like genes including several granzymes genes.
Conclusion: Expanded gammadelta T cells express Th1-like genes.
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| |
|
| Overall design |
Freshly isolated and IL-2 expanded gammadelta T cells, single samples, and total RNA was isolated and sequenced.
|
| Web link |
https://pubmed.ncbi.nlm.nih.gov/35843266/
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| |
|
| Contributor(s) |
Kujawski M, Dery K, Shively J |
| Citation(s) |
35843266 |
| |
| Submission date |
Aug 26, 2021 |
| Last update date |
Feb 26, 2024 |
| Contact name |
Bin Zhang |
| Organization name |
City of Hope
|
| Department |
Hem Malignancies Trans Sci
|
| Street address |
1218 S 5TH AVE
|
| City |
Monrovia |
| State/province |
CA |
| ZIP/Postal code |
91016 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA758112 |
| SRA |
SRP334337 |
