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Series GSE183625 Query DataSets for GSE183625
Status Public on Sep 14, 2021
Title single-cell analysis of experimental autoimmune orchitis (EAO) in mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Adult normal male testes (Nt) of mice and testes insulted with EAO (O50t,50 days after the first immunization) were profiled via scRNA-seq.
 
Overall design The induction of EAO were conducted 3 times of immunization with homogenized mouse testis along with Freund’s adjuvant with 14 days interval. Each time was followed by i.p. injection of 100 ng Bordetella pertussis toxin in 100 μl 1X PBS. The normal control group was named as N and its cells digested with trypsin/EDTA was thus named as Nt. The orchitic groups were named as O50 (50 days after the first immunization) and the cells of O50 digested with trypsin/EDTA was named as O50t. 3 unilateral decapsulated testes from 3 mice collected either from the group O50 or N were pooled for digestion.
 
Contributor(s) Li Y
Citation(s) 35111154
Submission date Sep 07, 2021
Last update date Feb 16, 2022
Contact name Yinchuan Li
E-mail(s) 18622397604@163.com, yinchuanli@aliyun.com
Organization name Nantong University
Department Medical School
Street address 16 wenfeng road, Chongchuan
City Nantong
State/province Jiangsu
ZIP/Postal code 226001
Country China
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (2)
GSM5563668 Nt
GSM5563669 O50t
Relations
BioProject PRJNA761421
SRA SRP336048

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE183625_RAW.tar 27.6 Mb (http)(custom) TAR (of TXT)
GSE183625_orchitis.rds.gz 1.5 Gb (ftp)(http) RDS
GSE183625_orchitis_barcodes.tsv.gz 30.6 Kb (ftp)(http) TSV
GSE183625_orchitis_counts.mtx.gz 70.8 Mb (ftp)(http) MTX
GSE183625_orchitis_genes.tsv.gz 165.8 Kb (ftp)(http) TSV
GSE183625_orchitis_meta.data.csv.gz 177.4 Kb (ftp)(http) CSV
GSE183625_orchitis_normalized_scale.data.mtx.gz 214.1 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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