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Series GSE183949 Query DataSets for GSE183949
Status Public on Sep 14, 2021
Title A TALE homeodomain transcription factors-binding protein recruits Polycomb Repressive Complex 2 to a subset of loci in rice shoot apical meristem [ChIP-seq]
Organism Oryza sativa Japonica Group
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In this work, we investigated the function of PAP1 (LOC_Os03g01840) in gene expression, H3K27 tri-methylation, and Polycomb Repressive Complex 2 (PRC2) binding by high-throughput approaches. The PAP1 loss-of-function results in defects of meristem development and gene expression similar to those of PRC2 and/or KNOX gene mutants and decreases of H3K27me3 and PRC2 binding at KNOX-binding genes in the meristem. PAP1-meidated PRC2 recruitment may contribute to H3K27me3 shifting from heterochromatin silencing to developmental gene repression during land plant evolution.
 
Overall design Examination of H3K27me3 modifications and EMF2b binding in wild type and pap1 mutant in rice.
 
Contributor(s) Tan FQ, Zhou DX
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Submission date Sep 11, 2021
Last update date Sep 16, 2021
Contact name Fengquan Tan
E-mail(s) tanfengquan@webmail.hzau.edu.cn
Organization name HZAU
Street address Hongshan
City Wuhan
ZIP/Postal code 430070
Country China
 
Platforms (1)
GPL30619 MGISEQ-2000RS (Oryza sativa Japonica Group)
Samples (10)
GSM5574753 ZH11 H3K27me3-rep1
GSM5574754 ZH11 H3K27me3-rep2
GSM5574755 pap1 H3K27me3-rep1
This SubSeries is part of SuperSeries:
GSE183951 A TALE homeodomain transcription factors-binding protein recruits Polycomb Repressive Complex 2 to a subset of loci in rice shoot apical meristem
Relations
BioProject PRJNA762474
SRA SRP336644

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE183949_RAW.tar 1.3 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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