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Series GSE184076 Query DataSets for GSE184076
Status Public on Sep 17, 2021
Title Crotamiton derivative JM03 extends lifespan and improves 1 oxidative and hypertonic stress resistance in Caenorhabditis 2 elegans via inhibiting OSM-9
Organism Caenorhabditis elegans
Experiment type Expression profiling by high throughput sequencing
Summary While screening our in-house 1,072 marketed drugs for their ability to extend the lifespan using Caenorhabditis elegans (C. elegans) as an animal model, crotamiton (N-ethyl-o-crotonotoluidide) showed anti-aging activity and was selected for further structural optimization. After replacing the ortho-methyl of crotamiton with ortho-fluoro, crotamiton derivative JM03 was obtained and showed better activity in terms of lifespan-extension and stress resistance than crotamiton. It was further explored that JM03 extended the lifespan of C. elegans through osmotic avoidance abnormal-9 (OSM-9). Besides, JM03 improves the ability of nematode to resist oxidative stress and hypertonic stress through OSM-9, but not osm-9/capsaicin receptor related-2 (OCR-2). Then the inhibition of OSM-9 by JM03 reduces the aggregation of Q35 in C. elegans via upregulating the genes associated with proteostasis. SKN-1 signaling was also found to be activated after JM03 treatment, which might contribute to proteostasis, stress resistance and lifespan extension. In summary, this study explored a new small molecule derived from crotamiton, which has efficient anti-oxidative, anti-hypertonic and anti-aging effects, and could further lead to promising application prospects.
Overall design Examination of JM03-treated and control group in C. elegans by RNA-seq, in triplicate
Contributor(s) Keting B, Jiali F, Wenwen L, Zelan H, Jian L
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Submission date Sep 14, 2021
Last update date Sep 17, 2021
Contact name Liu Wenwen
Phone 86+18930703292
Organization name East China University of Science and Technology
Street address 130 Meilong Road
City Shanghai
ZIP/Postal code 200237
Country China
Platforms (1)
GPL18245 Illumina HiSeq 2500 (Caenorhabditis elegans)
Samples (6)
GSM5577227 Ctrl rep1
GSM5577228 Ctrl rep2
GSM5577229 Ctrl rep3
BioProject PRJNA763103
SRA SRP336979

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Supplementary file Size Download File type/resource
GSE184076_JM03_treatment.xlsx 2.5 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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