NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE184076 Query DataSets for GSE184076
Status Public on Sep 17, 2021
Title Crotamiton derivative JM03 extends lifespan and improves 1 oxidative and hypertonic stress resistance in Caenorhabditis 2 elegans via inhibiting OSM-9
Organism Caenorhabditis elegans
Experiment type Expression profiling by high throughput sequencing
Summary While screening our in-house 1,072 marketed drugs for their ability to extend the lifespan using Caenorhabditis elegans (C. elegans) as an animal model, crotamiton (N-ethyl-o-crotonotoluidide) showed anti-aging activity and was selected for further structural optimization. After replacing the ortho-methyl of crotamiton with ortho-fluoro, crotamiton derivative JM03 was obtained and showed better activity in terms of lifespan-extension and stress resistance than crotamiton. It was further explored that JM03 extended the lifespan of C. elegans through osmotic avoidance abnormal-9 (OSM-9). Besides, JM03 improves the ability of nematode to resist oxidative stress and hypertonic stress through OSM-9, but not osm-9/capsaicin receptor related-2 (OCR-2). Then the inhibition of OSM-9 by JM03 reduces the aggregation of Q35 in C. elegans via upregulating the genes associated with proteostasis. SKN-1 signaling was also found to be activated after JM03 treatment, which might contribute to proteostasis, stress resistance and lifespan extension. In summary, this study explored a new small molecule derived from crotamiton, which has efficient anti-oxidative, anti-hypertonic and anti-aging effects, and could further lead to promising application prospects.
 
Overall design Examination of JM03-treated and control group in C. elegans by RNA-seq, in triplicate
 
Contributor(s) Keting B, Jiali F, Wenwen L, Zelan H, Jian L
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Sep 14, 2021
Last update date Sep 17, 2021
Contact name Liu Wenwen
E-mail(s) Y12180421@mail.ecust.edu.cn
Phone 86+18930703292
Organization name East China University of Science and Technology
Street address 130 Meilong Road
City Shanghai
ZIP/Postal code 200237
Country China
 
Platforms (1)
GPL18245 Illumina HiSeq 2500 (Caenorhabditis elegans)
Samples (6)
GSM5577227 Ctrl rep1
GSM5577228 Ctrl rep2
GSM5577229 Ctrl rep3
Relations
BioProject PRJNA763103
SRA SRP336979

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE184076_JM03_treatment.xlsx 2.5 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap